基于碱性和酸性氨基酸比值的陈化烤烟烟叶适用时间判断  

Suitable Quality Determination for Aged Flue-cured Tobacco Strips Based on Ratio of Basic Amino Acid to Acidic Amino Acid

作　　者：周显升[1] 周金辉 魏玉磊[1] 刘群[1] 王银龙[1] ZHOU Xiansheng;ZHOU Jinhui;WEI Yulei;LIU Qun;WANG Yinlong(Technology Center,China Tobacco Shandong Industrial Co.,Ltd.,Jinan 250014,China;Jinan Cigarette Factory,China Tobacco Shandong Industrial Co.,Ltd.,Jinan 250104,China)

机构地区：[1]山东中烟工业有限责任公司技术中心,济南250014 [2]山东中烟工业有限责任公司济南卷烟厂,济南250104

出　　处：《中国烟草科学》2023年第5期86-93,102,共9页Chinese Tobacco Science

基　　金：山东中烟工业有限责任公司重点科技项目(2007027)。

摘　　要：为明确陈化期间烟叶碱性和酸性氨基酸含量及其比值变化规律,并依据其比值判断陈化烟叶适宜使用时间,对福建龙岩、福建南平、贵州毕节、河南三门峡、湖南永州、四川凉山、陕西安康、云南保山、云南曲靖、山东、重庆和津巴布韦12个产区2006—2008年度烤烟复烤片烟样品定期取样,采用液相色谱法,跟踪分析了样品陈化期间游离碱性和酸性氨基酸变化,并分析了其与陈化烟叶感官质量的关系。结果发现:1.感官评吸结果表明,同一产区同一等级不同年度烟叶感官质量达到最优所需陈化时间不同;2.陈化期间碱性、酸性氨基酸含量比值逐渐降低,与陈化时间具有较好的线性相关关系,与感官质量具有较好的二次相关关系;3.应用2007、2008年度线性回归方程计算得出陈化烟叶适宜使用时间内的碱性、酸性氨基酸比值范围,经2006年度样品验证,判断准确率达85%。综上,碱性、酸性氨基酸比值可衡量烟叶陈化程度,为判断陈化烟叶质量提供了新方法。It is crucial to investigate the changes of contents and ratio of basic and acidic amino acids in aging process,and the ratio can be used to assess the quality of aged tobacco strips.Aiming to analyze these changes and their relationship with sensory quality,a study was conducted by using liquid chromatography to examine the variations in free basic and acidic amino acids of flue-cured tobacco strips in 2006―2008 from 12 regions,i.e.Longyan and Nanping(Fujian Province),Bijie(Guizhou Province),Sanmenxia(Henan Province),Yongzhou(Hunan Province),Liangshan(Sichuan Province),Ankang(Shaanxi Province),Baoshan and Qujing(Yunnan Province),Shandong,Chongqing,and Zimbabwe.Sensory evaluation results showed that aging time required for the peak sensory quality differed among the same tobacco grades produced in different years from the same region.In aging process,the ratio of basic amino acid content to acidic amino acid content gradually decreased,demonstrating a strong linear correlation with aging time and a significant correlation with sensory quality.Linear regression equations were adopted to calculate the range of basic and acidic amino acid ratios suitable for high-quality aged tobacco leaves in 2007 and 2008.After validating the samples in 2006,the judgment accuracy reached 85%.The ratio of basic to acidic amino acids can definitely measure the aging degree of tobacco leaves,which might provide a novel method for evaluating aging quality.

关 键 词：烤烟片烟 陈化 碱性氨基酸 酸性氨基酸 适用时间 

分 类 号：TS411[农业科学—烟草工业]