基于西洋参总皂苷调控TGF-β1/Smad信号通路对压力性尿失禁大鼠模型盆底功能恢复的影响  

Effect of TGF-β/Smad signaling pathway regulated by total saponins of American ginseng on functional recovery of pelvic floor in rats with stress urinary incontinence

作　　者：李露[1] 李秀娟[1] LI Lu;LI Xiujuan(Department of Obstetrics,Union Medical College Jiangbei Hospital,Huazhong University of Science and Technology,Wuhan 430100,China)

机构地区：[1]华中科技大学协和江北医院产科,武汉430100

出　　处：《实用医学杂志》2023年第19期2450-2455,共6页The Journal of Practical Medicine

基　　金：湖北省自然科学基金计划项目(编号:2018CFB243)。

摘　　要：目的探究基于西洋参总皂苷调控TGF-β1/Smad信号通路对压力性尿失禁大鼠模型盆底功能恢复的影响。方法选取65只SPF级SD大鼠,将建模完成的45只大鼠,随机分作模型组、西洋参总皂苷低、高剂量组各15只,剩余的15只未做任何处理的大鼠作对照组。低剂量西洋参总皂苷组及高剂量西洋参总皂苷组的大鼠分别依据50、100 mg/kg的用量进行灌服西洋参总皂苷,对照组、模型组实施同等量的生理盐水(15 mL)予以灌服。比较各组大鼠病理组织学、盆底功能相关指标、尿动力学有关指标、膀胱中内压有关指标、盆底肌肉表面电信号以及TGF-β1/Smad信号通路蛋白相对表达量。结果与对照组对比,模型组、西洋参总皂苷低、高剂量组的大鼠尿道旋转角度、膀胱颈的活动度、残余尿量、排尿量、Smad2、Smad3表达升高,阴道静息压、收缩压、LPP、MBC、ALPP、排尿效率、Ⅰ类及Ⅱ类盆底肌纤维电位、TGF-β1、Smad7水平下降(P<0.05);与模型组相比,西洋参总皂苷低、高剂量组大鼠的阴道静息压、收缩压、LPP、MBC、ALPP、排尿效率、Ⅰ类及Ⅱ类盆底肌纤维电位、TGF-β1、Smad7表达上升,尿道旋转角度、膀胱颈活动度、残余尿量、排尿量、Smad2、Smad3水平减少(P<0.05)。结论西洋参总皂苷可有效改善压力性尿失禁模型大鼠的盆底功能,其作用机制可能与TGF-β1/Smad信号通路有关。Objective To explore the effect of TGF-β1/Smad signaling pathway regulated by total saponins of American ginseng on the functional recovery of pelvic floor in rats with stress urinary incontinence.Methods Sixty-five SD rats of SPF grade were selected,and 45 rats after modeling were randomly divided into model group,low-dose group and high-dose group with 15 rats each,and the remaining 15 rats without any treatment were used as control group.Rats in low-dose group and high-dose group were given total saponins of Panax quinquefolium according to the dosage of 50 mg/kg and 100 mg/kg respectively,while rats in control group and model group were given the same amount of physiological saline(15 mL).The pathological histology,pelvic floor function related indexes,urodynamics related indexes,bladder internal pressure related indexes,pelvic floor muscle surface electrical signals and the relative expression of TGF-β1/Smad signaling pathway protein were compared in each group.Results Compared with those in the control group,urethra rotation angle,bladder neck motion,residual urine volume,urine output,Smad2 and Smad3 expression were increased in the model group,the low and high dose group;vaginal resting pressure,systolic pressure,LPP,MBC,ALPP,urinary efficiency,ClassⅠand ClassⅡpelvic floor muscle fiber potential,TGF-β1,and Smad7 level were decreased(P<0.05);Compared with those in the model group,vaginal resting pressure,systolic blood pressure,LPP,MBC,ALPP,urinary efficiency,classⅠand ClassⅡpelvic floor muscle fiber potential,TGF-β1,Smad7 expression increased in the low and high dose group;urethral rotation angle,bladder neck motion,residual urine volume,urine output,Smad2 and Smad3 levels were decreased(P<0.05).Conclusion American ginseng total saponin could effectively improve 19pelvic floor function in rats with stress urinary incontinence,and the mechanism may be related to TGF-β1/Smad signaling pathway.

关 键 词：压力性尿失禁 西洋参总皂苷 转化生长因子-β1/Smad 盆底功能 

分 类 号：R361[医药卫生—病理学]