鹅掌柴蜂蜜中特征性成分鉴定及真实性评价  

Characteristic Compounds Identification and Authenticity Evaluation of Heptapleurum Honey

作　　者：王琪琦 黄忠连 王莉 董捷[2] 林珣 张根生[1] WANG Qiqi;HUANG Zhonglian;WANG Li;DONG Jie;LIN Xun;ZHANG Gensheng(College of Food Engineering,Harbin University of Commerce,Harbin 150000,China;Institute of Apicultural Research,Chinese Academy of Agricultural Sciences,Beijing 100093,China;Tianmijia Bee Industry Co.,Ltd.,Wuzhou 543000,China;Jiangsu Hongqi Biotechnology Co.,Ltd.,Taizhou 225300,China)

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150000 [2]中国农业科学院蜜蜂研究所,北京100093 [3]广西梧州甜蜜家蜂业有限公司,广西梧州543000 [4]江苏鸿祺生物科技有限公司,江苏泰州225300

出　　处：《食品工业科技》2023年第23期238-245,共8页Science and Technology of Food Industry

基　　金：中国农业科学院科技创新工程项目(CAAS-ASTIP-2015 IAR);国家蜂产业技术体系项目(CARS-44-KXJ19)。

摘　　要：为明确鹅掌柴蜂蜜中特征性成分并建立鹅掌柴蜂蜜的真实性评价方法,本研究采用高效液相色谱-四极杆飞行时间串联质谱(HPLC-Q-TOF-MSMS)法对鹅掌柴蜂蜜中特征性成分进行定性定量分析。在鹅掌柴蜂蜜中共鉴定出5个成分,分别为4-(1’-环乙醚-3’-丁二醇)-3,5,5-三甲基-2-环己烯酮(苦蜜素B)、3,4,5-三甲氧基苯丙烯醇、4-(1’2’-二羟基-3’环氧丙烷)-3,5,5-2-环己烯酮(苦蜜素C)、反,反式脱落酸、顺,反式脱落酸。其中首次在蜂蜜中发现3,4,5-三甲氧基苯丙烯醇,并将其定为鹅掌柴蜂蜜的标志性成分。收集10个不同产地的鹅掌柴原料蜜,建立鹅掌柴蜂蜜的HPLC指纹图谱,结合特征性成分和指纹图谱对市售的6个鹅掌柴蜂蜜进行真实性评价,结果表明,其中3个品牌为品质较好的鹅掌柴蜂蜜,2个品牌为鹅掌柴蜂蜜中可能混入其他蜂蜜,1个品牌中未检测到鹅掌柴蜂蜜的标志性成分,并且色谱图与指纹图谱存在明显差异,推测其不是鹅掌柴蜂蜜。本研究为鹅掌柴蜂蜜真实性评价提供理论参考。In order to clarify the characteristic compounds and establish the authenticity evaluation method of heptapleurum honey,high-performance liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry(HPLCQ/TOF-MS/MS)was used to qualitative and quantitative analysis of characteristic compounds in heptapleurum honey.Five compounds were identified including 4-(1'-cyclodiethyl ether-3'-butanediol)-3,5,5-trimethyl-2-cyclohexenone(Unedone B),3,4,5-trimethoxy cinnamyl alcohol,4-(1'2'-dihydroxy-3'epoxypropane)-3,5,5-2-cyclohexenone(Unedone C),trans,trans abscisic acid,and cis,trans abscisic acid.Notably,3,4,5-trimethoxy cinnamyl alcohol was first found in honey,and it could be a marker of heptapleurum honey.Moreover,10 raw heptapleurum honey samples with different geographic origins were collected to establish HPLC fingerprint.The authenticity of six commercial heptapleurum honey samples were evaluated by combining characteristic compound with HPLC fingerprint.The results demonstrated that three brands of sample were genuine pure heptapleurum honey,two brands of sample might be mixed with other honeys.A brand sample did not contain characteristic compound and were significantly different with the standard fingerprints of heptapleurum honey.It was infe-rred that the sample was not heptapleurum honey.This study provides theoretical reference for the authenticity evaluation of heptapleurum honey.

关 键 词：鹅掌柴蜂蜜 特征性成分 指纹图谱 真实性评价 

分 类 号：S896.1[农业科学—特种经济动物饲养]