Environmental selection and evolutionary process jointly shape genomic and functional profiles of mangrove rhizosphere microbiomes  

作　　者：Xiaoli Yu Qichao Tu Jihua Liu Yisheng Peng Cheng Wang Fanshu Xiao Yingli Lian Xueqin Yang Ruiwen Hu Huang Yu Lu Qian Daoming Wu Ziying He Longfei Shu Qiang He Yun Tian Faming Wang Shanquan Wang Bo Wu Zhijian Huang Jianguo He Qingyun Yan Zhili He 

机构地区：[1]State Key Laboratory for Biocontrol,Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),School of Environmental Science and Engineering,Environmental Microbiomics Research Center,Sun Yat‐sen University,Guangzhou,China [2]Institute of Marine Science and Technology,Shandong University,Qingdao,China [3]College of Forestry&Landscape Architecture,South China Agricultural University,Guangzhou,China [4]Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),School of Marine Science,Sun Yat‐sen University,Guangzhou,China [5]Department of Civil and Environmental Engineering,The University of Tennessee,Knoxville,Tennessee,USA [6]Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems,School of Life Sciences,Xiamen University,Xiamen,China [7]Xiaoliang Research Station for Tropical Coastal Ecosystems and Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,China [8]School of Life Sciences,Sun Yat‐sen University,Guangzhou,China

出　　处：《mLife》2023年第3期253-266,共14页微生物（英文）

基　　金：supported by the Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)(SML2020SP004);the National Natural Science Foundation of China(91951207,32100077,31770539,31870469,42177011,41676105,41771095,62170346);the China Postdoctoral Science Foundation(2021M703751).

摘　　要：Mangrove reforestation with introduced species has been an important strategy to restore mangrove ecosystem functioning.However,how such activities affect microbially driven methane(CH4),nitrogen(N),and sulfur(S)cycling of rhizosphere microbiomes remains unclear.To understand the effect of environmental selection and the evolutionary process on microbially driven biogeochemical cycles in native and introduced mangrove rhizospheres,we analyzed key genomic and functional profiles of rhizosphere microbiomes from native and introduced mangrove species by metagenome sequencing technologies.Compared with the native mangrove(Kandelia obovata,KO),the introduced mangrove(Sonneratia apetala,SA)rhizosphere microbiome had significantly(p<0.05)higher average genome size(AGS)(5.8 vs.5.5 Mb),average 16S ribosomal RNA gene copy number(3.5 vs.3.1),relative abundances of mobile genetic elements,and functional diversity in terms of the Shannon index(7.88 vs.7.84)but lower functional potentials involved in CH4 cycling(e.g.,mcrABCDG and pmoABC),N2 fixation(nifHDK),and inorganic S cycling(dsrAB,dsrC,dsrMKJOP,soxB,sqr,and fccAB).Similar results were also observed from the recovered Proteobacterial metagenome-assembled genomes with a higher AGS and distinct functions in the introduced mangrove rhizosphere.Additionally,salinity and ammonium were identified as the main environmental drivers of functional profiles of mangrove rhizosphere microbiomes through deterministic processes.This study advances our understanding of microbially mediated biogeochemical cycling of CH_(4),N,and S in the mangrove rhizosphere and provides novel insights into the influence of environmental selection and evolutionary processes on ecosystem functions,which has important implications for future mangrove reforestation.

关 键 词：average genome size functional potential mangrove rhizosphere METAGENOME metagenome-assembled genome 

分 类 号：Q93[生物学—微生物学]