白细胞介素-8拮抗剂通过抑制活性氧生成下调视网膜血管内皮细胞黏附和迁移  

Interleukin-8 antagonist down regulates the adhesion and migration of retinal vascular endothelial cells by inhibiting the production of reactive oxygen species

作　　者：王林妮 曹靖靖 邢东军 于荣国 胡立影 杨旸 李嫦 李志清 李辉 洪亚茹 东莉洁 Wang Linni;Cao Jingjing;Xing Dongjun;Yu Rongguo;Hu Liying;YYang Yang;Li Chang;Li Zhiqing;Li Hui;Hong Yaru;Dong Lijie(Tianjin Medical University Eye Hospital,Eye Institute and School of Optometry,Tianjin Branch of National Clinical Research Center for Ocular Disease,Tianjin Key Laboratory of Retinal Functions and Diseases,Tianjin 300384,China)

机构地区：[1]天津医科大学眼科医院、眼视光学院、眼科研究所、国家眼耳鼻喉疾病临床医学研究中心、天津市分中心、天津市视网膜功能与疾病重点实验室,天津300384

出　　处：《中华眼底病杂志》2023年第11期913-917,共5页Chinese Journal of Ocular Fundus Diseases

基　　金：天津市高等教育委员会科技发展基金项目(2022ZD057);天津市滨海新区卫生健康委员会科技项目(2022BWKZ003);天津市视网膜功能与疾病重点实验室开放项目(2021tjswmm002);天津市卫生健康科研项目(TJWJ2023ZD002);天津市医学重点学科(专科)建设项目(TJYXZDXK-037A)。

摘　　要：目的观察白细胞介素-8(IL-8)对视网膜血管内皮细胞(RCEC)黏附和迁移的影响。方法细胞实验研究。将人RCEC(hRCEC)分为正常对照组(N组)、糖基化终末产物(AGE)处理组(AGE组)、AGE诱导联合IL-8抑制剂SB225002处理组(AGE+SB组)。采用蛋白质免疫印迹法观察AGE对hRCEC中IL-8表达水平的影响;细胞划痕实验观察SB225002对hRCEC迁移的影响;流式细胞仪检测SB225002对hRCEC上白细胞黏附、活性氧(ROS)产生的影响。两组间比较行Student-t检验;三组间比较行单因素方差分析。结果与N组比较,AGE组细胞中IL-8表达水平显著升高,差异有统计学意义(t-25.661,P<0.001)。与N组、AGE+SB组比较,AGE组细胞迁移率显著升高(F=29.776),白细胞黏附数量明显增多(F=38.159、38.556),ROS表达水平显著增高(F=22.336),差异均有统计学意义(P<0.05)。结论IL-8拮抗剂SB225002可能通过抑制ROS的表达而下调hRCEC的黏附和迁移。Objective To observe the effect of interleukin-8(IL-8)on the adhesion and migration of retinal vascular endothelial cells(RCEC).Methods A cell experiment.Human RCEC(hRCEC)was divided into normal control group(N group),advanced glycation end product(AGE)treatment group(AGE group),and AGE-induced combined IL-8 antagonist SB225002 treatment group(AGE+SB group).The effect of AGE on IL-8 expression in hRCEC was observed by Western blot.The effect of SB225002 on hRCEC migration was observed by cell scratch assay.The effects of SB225002 on leukocyte adhesion and reactive oxygen species(ROS)on hRCEC were detected by flow cytometry.Student-t test was performed between the two groups.Oneway analysis of variance was performed among the three groups.Results Compared with group N,the expression level of IL-8 in cells of AGE group was significantly increased,with statistical significance(t=25.661,P<0.001).Compared with N group and AGE+SB group,cell mobility in AGE group was significantly increased(F-29.776),leukocyte adhesion number was significantly increased(F-38.159,38.556),ROS expression level was significantly increased(F=22.336),and the differences were statistically significant(P<0.05).Conclusion IL-8 antagonist SB225002 may down-regulate hRCEC adhesion and migration by inhibiting ROS expression.

关 键 词：视网膜血管内皮细胞 白细胞介素-8 细胞迁移 细胞黏附 活性氧 细胞实验 

分 类 号：R774.1[医药卫生—眼科]