破乳诱导萃取食用植物油中营养元素的原子发射光谱分析  

Determination of nutrient elements in edible vegetable oil by atomic emission spectroscopy based on extraction induced by emulsion breaking

作　　者：李爱阳[1] 陈宇 殷子懿 黄建华 LI Aiyang;CHEN Yu;YIN Ziyi;HUANG Jianhua(Department of Chemical and Environmental Engineering,Hunan Institute of Technology,Hengyang 421002,China;Institute of Chinese Materia,Hunan Academy of Traditional Chinese Medicine,Changsha 410013,China)

机构地区：[1]湖南工学院化学与环境工程学院,衡阳421002 [2]湖南省中医药研究院中药研究所,长沙410013

出　　处：《分析试验室》2023年第10期1292-1298,共7页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(81603400);湖南省教育厅重点项目(20A140);湖南省应用特色学科材料科学与工程学科(湘教通[2018]469号);湖南省大学生创新训练项目(3980)资助。

摘　　要：基于破乳诱导萃取,提出利用微波等离子体原子发射光谱(MP-AES)测定食用植物油中多种营养元素的新策略。采用5%(w/V)的Triton X-114为乳化剂,50%(V/V)的HCl为萃取剂,对食用植物油样品于90℃下超声水浴40 min,将分析元素从有机基质转移至酸性水相,采用MP-AES测定其中的8种营养元素(K,Na,Ca,Mg,Fe,Mn,Cu,Zn)。8种元素在各自质量浓度范围内呈良好的线性关系,线性相关系数(R)≥0.9998,检出限(LOD)在0.16~1.95μg/L之间,样品的加标回收率在94.0%~105.0%之间,相对标准偏差(RSD)≤4.0%。本方法测定结果与国标法基本一致。应用所建立的方法对市场上不同产地的花生油、玉米油、油菜籽油、山茶油、葵花籽油和橄榄油进行检测,6种食用植物油中富含8种营养元素。本方法已用于食用植物油中营养元素的质量评价。A new strategy for the determination of multiple nutrient elements in edible vegetable oil using microwave plasma atomic emission spectroscopy(MP-AES) was proposed based on the extraction induced by emulsion breaking.Using 5%(w/V) Triton X-114 as emulsifier and 50%(V/V) hydrochloric acid as extractant,the edible vegetable oil samples were subjected to ultrasonic water bath at 90 ℃ for 40 min,leading to the analytes transferred from organic matrix to acidic aqueous phase.The eight kinds of nutrient elements(K,Na,Ca,Mg,Fe,Mn,Cu and Zn) were determined by MP-AES.The linear correlation coefficients of the analytes in their respective mass concentration ranges were no less than 0.9998,showing good linear relationships.The limits of detection(LODs) were 0.16-1.95 μg/L,and the spiked recoveries were 94.0%-105.0%,with the relative standard deviations(RSDs) no higher than 4.0%.The results of this method were basically consistent with that of the standard method.The developed method was used to detect nutrient elements in peanut oil,corn oil,rapeseed oil,camellia oil,sunflower oil and olive oil from different origins in the domestic market.The results showed 6 kinds of edible vegetable oils were rich in 8 kinds of nutrient elements.The method has been used for the quality evaluation of nutrient elements in edible vegetable oil.

关 键 词：破乳诱导萃取 食用植物油 微波等离子体原子发射光谱 营养元素 

分 类 号：O657.31[理学—分析化学]