低共熔溶剂液液微萃取-高效液相色谱法测定奶茶中3种甲基黄嘌呤类物质  

Deep eutectic solvent based liquid liquid microextraction-high performance liquid chromatography for the determination of three kinds of methylxanthines in milky tea

作　　者：张悦 杨楠 赵思睿 徐凡 谢哲浩 刘彦[1] ZHANG Yue;YANG Nan;ZHAO Sirui;XU Fan;XIE Zhehao;LIU Yan(School of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China)

机构地区：[1]河北医科大学药学院,石家庄050017

出　　处：《分析试验室》2023年第10期1351-1356,共6页Chinese Journal of Analysis Laboratory

基　　金：2022年河北省医学科学研究课题计划(20220080);河北医科大学2021年大学生创新性实验计划项目(USIP2021282)资助。

摘　　要：建立了基于低共熔溶剂(DES)的液液微萃取-高效液相色谱法(HPLC)测定奶茶中可可碱、咖啡因和茶碱含量的方法。制备了8种不同组成的DES,并选择百里酚-辛酸摩尔比为2∶1的DES为萃取溶剂。在优化的样品前处理及色谱条件下,可可碱、咖啡因和茶碱分别在0.1~50.0μg/mL浓度范围内线性关系良好,相关系数(r)分别为0.9981,0.9988和0.9975,检出限(LOD)分别为0.06,0.05,0.06μg/mL,加标回收率为93.8%~102.7%,相对标准偏差(RSD)≤3.9%。该方法适用于测定奶茶中可可碱、咖啡因和茶碱的含量。A method of liquid-liquid microextraction(LLME) based on deep eutectic solvent(DES) combined with high performance liquid chromatography(HPLC) was established to detect the contents of theophylline,theobromine and caffeine in milky tea.8 kinds of DESs with different composition were prepared,and DES with the molar ratio of 5-methyl-2-isopropylphenol to octanoic acid of 2∶1 was selected as the extraction solvent.Under the optimized conditions,there were good linear relationships of theophylline,theobromine and caffeine in the range of 0.1-50.0 μg/mL,with the correlation coefficients(r) of 0.9981,0.9988 and 0.9975,respectively.The limits of detection(LODs) of theophylline,theobromine and caffeine were 0.06,0.05 and 0.06 μg/mL,and the average recoveries were 93.8%-102.7%,respectively.With the relative standard deviations(RSDs) less than 3.9%.This method can be applied to detect the contents of three kinds of methylxanthines in milky tea.

关 键 词：低共熔溶剂 甲基黄嘌呤类 奶茶 液液微萃取 样品前处理 

分 类 号：X132[环境科学与工程—环境科学]