黄芩苷保护2型糖尿病大鼠的肠黏膜屏障损伤的作用及机制  

Protective effect and mechanism of baicalin on intestinal mucosal barrier injury in type 2 diabetic rats

作　　者：房春娟[1] 姜雯雯[1] 马丹丹[1] 熊茫茫 刘燕玲[1] FANG Chun-juan;JIANG Wen-wen;MA Dan-dan;XIONG Mang-mang;LIU Yan-ling(School of Medicine,Jiangxi University of Science and Technology,Nanchang 330098,Jiangxi Province,China)

机构地区：[1]江西科技学院医学院,江西南昌330098

出　　处：《中国临床药理学杂志》2023年第21期3121-3124,共4页The Chinese Journal of Clinical Pharmacology

基　　金：江西省教育厅科学技术研究基金资助项目(GJJ2202606)。

摘　　要：目的探讨黄芩苷在保护2型糖尿病大鼠肠黏膜屏障损伤方面的作用机制。方法将雄性SD大鼠随机分为正常对照组(给予低脂饮食,经口灌胃给予等量的0.9%NaCl)、模型组(高脂饮食联合30 mg·kg^(-1)链脲佐菌素,经口灌胃给予等量的0.9%NaCl)和黄芩苷组(模型建立成功后经口灌胃给予50 mg·kg^(-1)黄芩苷),给药4周。观察大鼠的代谢水平、肠黏膜屏障损伤、肠道微生物和炎症状态的变化。结果对照组、模型组和黄芩苷组大鼠血糖水平分别为(6.38±0.37)、(20.62±0.73)和(14.89±8.62)mmol·L^(-1),血清胰岛素分别为(21.03±0.92)、(17.06±0.65)和(19.08±0.52)mU·L^(-1),总胆固醇(TC)分别为(0.95±0.16)、(1.63±0.28)和(1.12±0.13)mmol·L^(-1),低密度脂蛋白胆固醇(LDL-C)分别为(0.76±0.13)、(1.03±0.05)和(0.85±0.02)mmol·L^(-1),白细胞介素-1β(IL^(-1)β)分别为(22.61±3.15)、(108.37±13.45)和(61.82±3.37)pg·mL^(-1),白细胞介素-6(IL-6)分别为(57.47±9.78)、(212.61±36.77)和(110.34±13.27)pg·mL^(-1),模型组上述指标与对照组比较,黄芩苷组与模型组比较,差异均有统计学意义(均P<0.05)。与模型组比较,黄芩苷组大鼠肠道菌群中屎肠球菌、乳酸双球菌和拟杆菌属的细菌数量均显著增加,黄芩苷组枯草芽孢杆菌、棒状杆菌的细菌数量均显著减少(均P<0.05)。结论黄芩苷可通过调节肠道微生物和抑制炎症来保护2型糖尿病大鼠的肠黏膜屏障,对于预防和治疗相关疾病具有潜在的临床应用价值。Objective This study aimed to investigate the mechanism of baicalin in protecting the intestinal mucosal barrier in type 2 diabetic rats.Methods Male SD rats were randomly divided into control group(given a low-fat diet and administered an equal amount of 0.9%NaCl by gavage),model group(fed a high-fat diet combined with 30 mg·kg^(-1)colistin and administered an equal amount of 0.9%NaCl by gavage),and baicalin group(given 50 mg·kg^(-1)baicalin by gavage after the model was established)for 4 weeks.Changes in metabolic levels,intestinal mucosal barrier injury,intestinal microbiota,and inflammatory status in rats were observed.Results The blood glucose levels of rats in the control group,model group,and baicalin group were(6.38±0.37),(20.62±0.73)and(14.89±0.62)mmol·L^(-1),respectively;and the serum insulin levels were(21.03±0.92),(17.06±0.65)and(19.08±0.52)mU.L^(-1),respectively;the total cholesterol(TC)levels were(0.95±0.16),(1.63±0.28)and(1.12±0.13)mmol·L^(-1),respectively,while the low-density lipoprotein cholesterol(LDL-C)levels were(0.76±0.13),(1.03±0.05)and(0.85±0.02)mmol·L^(-1),respectively;the levels of interleukin-1β(IL-1β)were(22.61±3.15),(108.37±13.45)and(61.82±3.37)pg·mL-,respectively,while the levels of interleukin-6(IL-6)were(57.47±9.78),(212.61±36.77)and(110.34±13.27)pg·mL^(-1),respectively.The diferences between the model group and the control group and between the baicalin group and the model group were statistically significant(all P<0.05).Compared to the model group,the number of bacteria in the intestinal flora of rats in the baicalin group increased significantly for Enterococcus faecalis,Lactobacillus lactis,and Bacteroides with decreased significantly for Bacillus subtilis,Corynebacterium(all P<0.05).Conclusion Baicalin can protect the intestinal mucosal barrier of type 2 diabetic rats by regulating intestinal microbiota and inhibiting inflammation,and has potential clinical application value for the prevention and treatment of related diseases.

关 键 词：黄芩苷 2型糖尿病 肠黏膜屏障 微生物组 炎症状态 

分 类 号：R28[医药卫生—中药学]