外周血淋巴细胞亚群在非小细胞肺癌中的表达及意义  

Expression and significance of peripheral blood lymphocyte subsets in non-small cell lung cancer

作　　者：赵皓[1] 齐学远[2] 王俊斌[3] 张小凤 陶露 余晾[1] ZHAO Hao;QI Xueyuan;WANG Junbin;ZHANG Xiaofeng;TAO Lu;YU Liang(Central Laboratory,the First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui 233004,China;不详)

机构地区：[1]蚌埠医学院第一附属医院中心实验室,安徽蚌埠233004 [2]蚌埠医学院第一附属医院呼吸内科 [3]蚌埠医学院第一附属医院肿瘤内科

出　　处：《中华全科医学》2023年第11期1849-1851,1981,共4页Chinese Journal of General Practice

基　　金：安徽省重点研究与开发计划项目(202004j07020010)。

摘　　要：目的探讨不同病理类型及临床分期的非小细胞肺癌患者外周血中淋巴细胞亚群的动态变化及意义,对比非小细胞肺癌患者与健康人群的免疫状态。方法采用流式细胞术对蚌埠医学院第一附属医院2020年5月-2023年2月呼吸内科和肿瘤内科住院的非小细胞肺癌患者外周血淋巴细胞亚群进行检测,其中肺鳞状细胞癌55例、肺腺癌49例,另筛选同期健康体检中心25例健康成人作为对照。结果非小细胞肺癌组的CD3^(+)细胞、CD4^(+)细胞比例分别为(67.43±9.01)%、(37.34±9.15)%,CD4^(+)/CD8^(+)值为1.57±0.80,低于对照组,NK细胞比例为(21.22±8.06)%,相比对照组明显增高,差异有统计学意义(均P<0.05)。肺鳞状细胞癌与肺腺癌相比,各组数据差异均无统计学意义。Ⅲ~Ⅳ期CD3^(+)细胞、CD4^(+)细胞比例分别为(66.33±9.52)%、(36.01±9.44)%,低于Ⅰ~Ⅱ期,NK细胞比例为(22.81±8.43)%,相比Ⅰ~Ⅱ期明显增高,差异有统计学意义(均P<0.05)。结论在非小细胞肺癌的发生过程中患者免疫功能受到抑制,并且随着病情的进展这种抑制作用进一步加重,检测非小细胞肺癌患者外周血中T淋巴细胞亚群、B细胞和NK细胞的占比对了解机体免疫状况、评估病情及指导治疗具有一定的临床意义。Objective To investigate the dynamic changes and significance of lymphocyte subsets in peripheral blood of non-small cell lung cancer(NSCLC)patients with different pathological types and clinical stages,and to compare the immune status of non-small cell lung cancer patients with healthy people.Methods Peripheral blood lymphocyte subsets of non-small cell lung cancer patients admitted to the Respiratory and Oncology Departments of the First Affiliated Hospital of Bengbu Medical College from May 2020 to February 2023 were detected by flow cytometry,including 55 cases of lung squamous cell carcinoma and 49 cases of lung adenocarcinoma,and 25 healthy adults from the health examination center during the same period were screened as controls.Results Compared with the control group,the proportions of CD3^(+)cells and CD4^(+)cells in NSCLC group were(67.43±9.01)%and(37.34±9.15)%,the ratio of CD4^(+)/CD8^(+)was 1.57±0.80,which were lower than that in the control group;The proportion of NK cells was(21.22±8.06)%,which was significantly higher than that of the control group;The differences were statistically significant(all P<0.05).There was no significant difference between squamous cell carcinoma and adenocarcinoma.InⅢ-Ⅳperiod,the proportion of CD3^(+) cells and CD4^(+)cells were(66.33±9.52)%and(36.01±9.44)%,belowⅠ-Ⅱperiod;The proportion of NK cells was(22.81±8.43)%,which was significantly higher than that in stageⅠ-Ⅱ;The differences were statistically significant(all P<0.05).Conclusion During the occurrence of NSCLC,the immune function of patients is inhibited,and this inhibition is further aggravated with the progression of the disease.It has certain clinical significance to detect the proportion of T lymphocyte subsets,B cells and NK cells in peripheral blood of NSCLC patients to understand the immune status of the body,evaluate the disease and guide the treatment.

关 键 词：非小细胞肺癌 临床分期 淋巴细胞亚群 流式细胞术 NK细胞 

分 类 号：R734.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]