miR-433-3p靶向HP1BP3抑制胃癌细胞的增殖、迁移和侵袭能力  

miR-433-3p targets HP1BP3 to inhibit proliferation,migration and inva⁃sion of gastric cancer cells

作　　者：陈虹 崔婧 罗晨 李峰[2] CHEN Hong;CUI Jing;LUO Chen;LI Feng(Department of Biochemistry and Molecular Biology,Basic Medical College,Shanxi Medical University,Taiyuan 030001,China;Central Laboratory,Shanxi Cancer Hospital,Taiyuan 030012,China)

机构地区：[1]山西医科大学基础医学院生物化学与分子生物学教研室,山西太原030001 [2]山西省肿瘤医院中心实验室,山西太原030013

出　　处：《中国病理生理杂志》2023年第11期1964-1972,共9页Chinese Journal of Pathophysiology

基　　金：山西省卫健委重点攻关专项(No.2022XM28);山西省基础研究计划面上项目(No.20220302122128)。

摘　　要：目的:探究微小RNA-433-3p(miR-433-3p)对胃癌细胞增殖、迁移和侵袭的影响,以及miR-433-3p靶向异染色质蛋白1结合蛋白3(HP1BP3)对胃癌细胞的调控作用。方法:将人胃癌细胞系HGC-27和AGS分为阴性对照(NC)mimic组、miR-433-3p mimic组、NC siRNA(si-NC)组和HP1BP3 siRNA(si-HP1BP3)组,分别转染NC mimic、miR-433-3p mimic、si-NC和si-HP1BP3。RT-qPCR检测miR-433-3p在HGC-27和AGS细胞中的表达;CCK-8法和细胞集落形成实验检测细胞增殖;划痕愈合实验检测细胞迁移;Traswell实验检测细胞侵袭;Western blot检测HP1BP3蛋白表达。结果:与NC mimic组相比,miR-433-3p mimic组HGC-27和AGS细胞中miR-433-3p表达升高。过表达miR-433-3p抑制HGC-27和AGS细胞的增殖、迁移和侵袭,抑制HP1BP3蛋白表达;敲减HP1BP3抑制HGC-27和AGS细胞的增殖和迁移。结论:miR-433-3p靶向调控HP1BP3进而抑制胃癌细胞的增殖、迁移和侵袭。AIM:To explore the role of microRNA-433-3p(miR-433-3p)in the proliferation,migration and invasion of gastric cancer cells as well as how it targets heterochromatin protein 1 binding protein 3(HP1BP3).METHODS:Human gastric cancer AGS and HGC-27 cells were divided into negative control(NC)mimic group,miR-433-3p mimic group,NC siRNA(si-NC)group and HP1BP3 siRNA(si-HP1BP3)group,and NC mimic,miR-433-3p mimic,si-NC and si-HP1BP3 were transfected into the cells in each group,respectively.The expression of miR-433-3p was detected by RT-qPCR.The cell proliferation was evaluated by CCK-8 and colony formation assays.The cell migration and invasion were detected by wound-healing and Transwell assays,respectively.The HP1BP3 expression was confirmed by Western blot.RESULTS:Compared with NC mimic group,miR-433-3p expression was increased in miR-433-3p mimic group.Overexpression of miR-433-3p,which decreased the expression of HP1BP3,suppressed the proliferation,migration and invasion of gastric cancer cells.Knockdown of HP1BP3 inhibited the proliferation and migration of gastric cancer cells.CONCLUSION:miR-433-3p inhibited the proliferation,migration and invasion of gastric cancer by targeting HP1BP3.

关 键 词：胃癌 微小RNA-433-3p 异染色质蛋白1结合蛋白3 

分 类 号：R735.2[医药卫生—肿瘤] R363.2[医药卫生—临床医学]