基于RNA-seq技术分析德州驴不同部位肌肉的差异表达基因  

Screening of differentially expressed genes related to Dezhou donkey meat quality based on RNA-seq technology

作　　者：许静[1] 姚欢丰 程杰 王延涛 李泽宇 曲洪磊 李孟孟 朱明霞[1] 王长法 柴文琼 XU Jing;YAO Huanfeng;CHENG Jie;WANG Yantao;LI Zeyu;QU Honglei;LI Mengmeng;ZHU Mingxia;WANG Changfa;CHAI Wenqiong(College of Agronomy and Agricultural Engineering,Liaocheng University,Liaocheng 252000,China;National Engineering Research Center for Gelatin-based Traditional Chinese Medicine/Dong-E-E-JiaoCompanyLimited,Donge252201,China;College of Animal Science and Technology,China Agricultural University,Beijing 100193,China)

机构地区：[1]聊城大学农学与农业工程学院,山东聊城252000 [2]国家胶类中药工程技术研究中心/东阿阿胶股份有限公司,山东东阿252201 [3]中国农业大学动物科技学院,北京100193

出　　处：《扬州大学学报（农业与生命科学版）》2023年第5期71-78,共8页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金青年基金项目(32102531);聊城大学大学生创新创业训练计划项目(202110447018);聊城大学博士科研启动基金项目(318051920);聊城大学畜牧学学科开放课题(319312101-32);山东省驴产业科技协同创新中心(聊城大学)开放课题(319330807);山东省“泰山产业领军人才”项目(LJNY201713、LJNY202022)。

摘　　要：以德州驴为研究对象,利用转录组学(RNA-seq)技术对其背最长肌、腿后腱、臀大肌不同部位差异表达基因进行测序,筛选调控驴肉品质性状的关键基因,探究不同部位驴肉差异的分子机制。结果表明:筛选获得了臀大肌与背最长肌差异表达基因124个,其中上调基因73个,下调基因51个;腿后腱与背最长肌差异表达基因126个,其中上调基因68个,下调基因58个。对德州驴腿后腱与背最长肌差异基因KEGG通路富集分析发现,差异表达基因主要在赖氨酸降解、β-丙氨酸代谢、脂肪酸降解等通路中高度富集,臀大肌与背最长肌差异表达基因富集氧化磷酸化、亮氨酸和异亮氨酸降解等通路中。同时,肉品质相关候选基因GCDH、ALDH2、IGF1、VGLL2、TECRL、ASS1、ATP5A1、MUT、ACADSB参与肌肉氨基酸脂肪酸代谢过程,在肌肉生长过程中发挥着重要作用。Using Dezhou donkey as research objects, the transcriptome of donkey muscle in different parts was analyzed in order to obtain the key genes related to regulating meat quality traits and provide a theoretical basis for improving donkey meat quality. The results showed that there were 124 differential genes between gluteus and longissimus dorsi muscle, including 73 up-regulated genes and 51 down-regulated genes. There were 126 differential genes between leg muscle and longissimus dorsi muscle, including 68 up-regulated genes and 58 down-regulated genes. KEGG pathway enrichment analysis of the differential gene between Dezhou donkey leg muscle and longissimus dorsi muscle showed that the differentially expressed genes were highly enriched in lysine degradation, alanine metabolism, fatty acid degradation and other pathways, and the differentially expressed genes between gluteus muscle and longissimus dorsi muscle were enriched in oxidative phosphorylation,leucine and isoleucine degradation and other pathways.At the same time,the candidate genes GCDH,ALDH2,IGFI,VGLL2,TECRL,ASS1,ATP5A1,MUTand ACADSBrelated to meat quality were screened,which were involved in the metabolism of amino acids and fatty acids in muscle and played an important role in the process of muscle growth.

关 键 词：驴 RNA-SEQ 差异表达基因 肉质性状 骨骼肌 

分 类 号：S822.2[农业科学—畜牧学]