放疗抑制成釉细胞源性肿瘤细胞生长的机制  

Mechanism of radiotherapy inhibiting the growth of ameloblastogenic tumor cells

作　　者：孙强[1] 王攀攀 魏凯通 马雅晴 郑宏雨 孙明磊[1] 赵军方[1] 陈万涛[3] Sun Qiang;Wang Panpan;Wei Kaitong;Ma Yaqing;Zheng Hongyu;Sun Minglei;Zhao Junfang;Chen Wantao(Department of Oral and Maxillofacial Surgery,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Department of Oral and Maxillofacial Surgery,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400042,China;Department of Oral and Maxillofacial Surgery,the Ninth People’s Hospital Affiliated to Shanghai Jiaotong University School of Medicine,Shanghai 200025,China)

机构地区：[1]郑州大学第一附属医院口腔颌面外科,郑州450052 [2]重庆医科大学附属第一医院口腔颌面外科,重庆400042 [3]上海交通大学医学院附属第九人民医院口腔颌面外科,上海200025

出　　处：《中华实验外科杂志》2023年第10期2059-2064,共6页Chinese Journal of Experimental Surgery

基　　金：河南省自然科学基金面上项目(212300410391);河南省医学适宜技术推广项目(SYJS2022113);河南省科技厅重点研发与推广专项(科技攻关)项目(212102310592);河南省医学科技攻关计划联合共建项目(LHGJ20200270);河南省医学科技攻关(省部共建重点)项目(SBGJ202002071);河南省中青年卫生健康科技创新人才(杰青)项目(YXKC2020030)。

摘　　要：目的观察成釉细胞源性肿瘤对放射治疗的临床敏感性,通过细胞实验研究成釉细胞瘤细胞对放射线敏感的机制。方法收集2012年10月至2020年12月术后复发失去手术指征并经过放射治疗的6例成釉细胞源性肿瘤患者的基本资料和临床信息。以成釉细胞瘤细胞系AM-1为研究对象,相关统计学方法分析AM-1对放疗的敏感性,以及放疗对细胞生物学行为的影响。结果6例成釉细胞源性肿瘤患者接受了放射治疗,男4例,女2例。1例患者达到部分缓解,1例患者达到完全消失,4例患者疾病无进展。放疗对AM-1细胞生长有抑制作用。4 Gy放疗后,细胞凋亡率高于0 Gy组。4 Gy组经辐照后72 h,处于S期的细胞减少(P<0.05),G_(1)、G_(2)/M期的细胞增多(P<0.05)。细胞克隆形成能力也受到抑制。结论放射治疗成釉细胞源性肿瘤具有良好的临床治疗效果,并且在细胞学水平得到进一步证实。Objective To observe the clinical sensitivity of ameloblastogenic tumor to radiotherapy,and to study the mechanism of radiosensitivity of ameloblastoma cells by cell experiments.Methods The basic data and clinical information of 6 patients with ameloblastic tumor who lost surgical indications for postoperative recurrence and underwent radiation therapy from October 2012 to December 2020 were collected.The ameloblastoma cell line AM-1 was used to analyze the sensitivity of AM-1 to radiotherapy and the effect of radiotherapy on the biological behavior of the cells by relevant statistical methods.Results Six patients with ameloblastogenic tumors received radiation therapy,4 males and 2 females.One patient achieved a partial response,one patient achieved complete disappearance,and four patients had no disease progression.Radiotherapy inhibited AM-1 cell growth.After 4 Gy radiotherapy,the apoptosis rate was higher than that in the 0 Gy group.In the 4 Gy group,cells in S phase decreased(P<0.05)and cells in G_(1) and G_(2)/M phases increased(P<0.05)at 72 hours after irradiation.Cell clonogenic ability was also inhibited.Conclusion Radiation therapy for ameloblastogenic tumors has a good clinical therapeutic effect and has been further confirmed at the cytological level.

关 键 词：成釉细胞瘤 敏感性 放疗 成釉细胞癌 

分 类 号：R730.5[医药卫生—肿瘤]