Dalbergia odorifera Essential Oil Protects against Myocardial Ischemia through Upregulating Nrf2 and Inhibiting Caspase Signaling Pathways in Isoproterenol-Induced Rats  

作　　者：Can-Hong Wang Bao Gong Hui Meng Yu-Lan Wu Xiang-Sheng Zhao Jian-He Wei 

机构地区：[1]Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine,Hainan Branch of the Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Haikou,China [2]Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization,Hainan Branch of the Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Haikou,China [3]Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College [4]Ministry of Education and National Engineering Laboratory for Breeding of Endangered Medicinal Materials,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China

出　　处：《World Journal of Traditional Chinese Medicine》2023年第3期338-347,共10页世界中医药杂志（英文）

基　　金：supported by the Hainan Province Science and Technology Special Fund(ZDYF2023SHFZ141,ZDYF2018123,ZDYF2021SHFZ077);the CAMS Innovation Fund for Medical Sciences(2021-I2M-1-032);Natural Science Foundation of Hainan Province,China(2019RC344)。

摘　　要：Objective:Dalbergia odorifera has long been used as a Chinese herbal medicine for the treatment of cardiovascular and cerebrovascular diseases.This study aimed to determine the potential myocardial protective effect and possible mechanism of action of D.odor ife ra essential oil(DOEO).Materials and Methods:The essential oil of D.odorifera was extracted by hydrodistillation.The cardioprotective effects of DOEO were examined by histopathological observation,myocardial enzyme detection,peroxidation,anti-oxidant level detection,and related protein expression.The compounds in the blood were identified by gas chromatography-mass spectrometry.Results:These results showed that DOEO had significant myocardial cell protection,with IC50 values ranging from 17.64 to 24.78μg/mL in vitro.Compared to the myocardial ischemia group,the DOEO pretreatment groups had lower levels of myocardial injury,creatinine kinase,lactate dehydrogenase,alanine transaminase,aspartate transaminase,hydrogen peroxide,and nitric oxide,and higher levels of glutathione and superoxide dismutase.In addition,DOEO pretreatment significantly increased Na+-K+-ATPase and Ca2+-ATPase levels.Moreove r,immunohistochemical e xperiments showed that DOEO remarkably increased the protein levels of NF-E2-related nuclear factor 2(Nrf2)and heme oxygenase-1(HO-1)and reduced the expression of apoptotic caspases,including caspase 3 and caspase 9.The main components of the blood were transnerolidol and nerolidol oxide.Overall,the study showed that DOEO displayed myocardial protection by upregulating the NF-E2-related nuclear factor-antioxidant response element(Nrf2-ARE)and caspase pathways.DOEO has a therapeutic effect on MI by inhibiting the oxidant and apoptotic effects.Conclusions:D.odorifera may be a potential candidate drug for treating myocardial ischemic injury.

关 键 词：ANTI-APOPTOSIS ANTI-OXIDATION Dalbergia odorifera essential oil ISOPROTERENOL Myocardial ischemia 

分 类 号：R285.5[医药卫生—中药学]