鸡腿菇产纤溶酶液体发酵体系优化  

Optimization of liquid fermentation system for production of fibrinolytic enzyme from Coprinus comatus

作　　者：王今雨 刘晓兰 景言[2] 郑喜群 WANG Jinyu;LIU Xiaolan;JING Yan;ZHENG Xiqun(College of Food Engineering,Harbin University of Commerce,Harbin 150028,China;Key Laboratory of Corn Deep ProcessingTheory and Technology of Heilongjiang Province,College of Food and Bioengineering,Qiqihar University,Qiqihar 161006,China;College of Food,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150028 [2]齐齐哈尔大学食品与生物工程学院、黑龙江省玉米深加工理论与技术重点实验室,黑龙江齐齐哈尔161006 [3]黑龙江八一农垦大学食品学院,黑龙江大庆163319

出　　处：《中国酿造》2023年第11期189-196,共8页China Brewing

基　　金：黑龙江省重点研发计划指导类项目(GZ20210097);国家自然科学基金面上项目(31171744);黑龙江省自然科学基金重点项目(ZD201305);黑龙江省省属本科高校基本科研业务费项目(145209314)。

摘　　要：该研究采用液态发酵法发酵鸡腿菇,鸡腿菇纤溶酶活力为评价指标,通过单因素试验、Plackett-Burman试验及Box-Behnken试验对其培养基组成和培养条件进行优化。结果表明,最佳培养基的组成为果糖2.9%,豆饼粉3.6%,KH2PO_(4)0.25%,MgSO_(4)0.2%。最佳培养条件为装液量50 mL/250 mL,接种量10%,发酵温度24℃,发酵时间6 d,摇床转速160 r/min。在此优化培养基及培养条件下,纤溶酶活力为136.89 U/mL,比优化前提高了4.28倍。Coprinus comatus was fermented with liquid fermentation method,using fibrinolytic enzyme activity as the evaluation index,the medium composition and culture conditions were optimized by single factor experiments,Plackett-Burman experiments and Box-Behnken experiments.The results showed that the optimal medium composition was fructose 2.9%,soybean cake 3.6%,KH2PO_(4)0.25%,MgSO_(4)0.2%.The optimal culture conditions were liquid volume 50 ml/250 ml,inoculum 10%,fermentation temperature 24℃,time 6 d,and shaking speed 160 r/min.Under the optimized medium and culture conditions,the fibrinolytic enzyme activity was 136.89 U/ml,which was 4.28 times higher than that before optimization.

关 键 词：鸡腿菇 纤溶酶 液体发酵 培养基 培养条件 响应面优化 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]