响应面法优化紫穗槐叶多糖提取工艺及其抗氧化活性分析  

Optimization of extraction conditions by response surface methodology and antioxidant activity of polysaccharide from Amorpha fruticosa leaves

作　　者：刘懿娴[1] 赵莹[1] 陈慧平[1] 邹敏[1] LIU Yi-xian;ZHAO Ying;CHEN Hui-ping;ZOU Min

机构地区：[1]郑州大学医药科学研究院,河南郑州450052

出　　处：《饲料研究》2023年第22期69-73,共5页Feed Research

基　　金：河南省重点研发与推广专项(项目编号:232102310371);河南省医药科学研究院基本科研业务费项目(项目编号:2022BP0118)。

摘　　要：试验以紫穗槐叶为原料,采用超声辅助酶解法提取紫穗槐叶多糖。以多糖得率为指标,通过单因素试验确定纤维素酶浓度、酶作用时间和反应温度的取值范围。利用响应面试验优化超声辅助酶解法提取紫穗槐叶多糖的工艺条件。结果显示,紫穗槐叶多糖的最佳提取条件为纤维素酶浓度5%、酶作用时间2 h、反应温度50℃,多糖的实际提取率为8.31%,与理论模拟值8.40%接近,建立的模型真实可靠。抗氧化试验表明,紫穗槐叶多糖对DPPH自由基和羟基自由基均有较强的清除能力,最大清除率分别为83.79%和99.18%。紫穗槐叶多糖对脂质氧化也具有较强的抑制能力,对β-胡萝卜素-亚油酸氧化体系的抑制能力约为2,6-二叔丁基对甲酚(BHT)的83%。研究表明,响应面法优化紫穗槐叶多糖提取工艺稳定可行,且紫穗槐叶多糖具有较强的抗氧化活性,具有较高的应用价值。Using Amorpha fruticosa leaves as raw materials,the polysaccharides were extracted from Amorpha fruticosa leaves by ultrasonic-assisted enzymatic hydrolysis.The effect of enzyme concentration,enzymolysis time and enzymolysis temperature on the extraction rate of polysaccharides was investigated by single factor test.The technical conditions of ultrasonic-assisted enzymatic hydrolysis for extracting polysaccharide from Amorpha fruticosa leaves were optimized by response surface methodology.The results showed that Amorpha fruticosa leaf polysaccharide was finally determined as follows:The enzyme concentration of 5%,the incubation time of 2 h,and the reaction temperature of 50℃.The actual extraction rate of Amorpha fruticosa leaf polysaccharide was 8.31%,which was close to the theoretical simulation value of 8.40%,and the established model was true and reliable.The antioxidant experiments showed that the polysaccharides from Amorpha fruticosa leaves had a strong scavenging ability on DPPH radical and hydroxyl radical,with the maximum scavenging rates of 83.79%and 99.18%,respectively.The polysaccharide from Amorpha fruticosa leaves also had a strong inhibition on lipid oxidation,and its inhibition onβ-carotene-linoleic acid oxidation system was about 83%of that of BHT.The study indicates that the extraction conditions of polysaccharide from Amorpha fruticosa leaves optimized by response surface methodology are stable and feasible.The polysaccharide has strong antioxidant activity and has high application value.

关 键 词：紫穗槐叶 多糖 提取 响应面 抗氧化活性 

分 类 号：S816.7[农业科学—饲料科学]