基于大科学装置的纳米材料与生物分子相互作用的分析  

Progress in analytical methods for the interaction between nanomaterials and biomolecules based on large-scale scientific facilities

作　　者：郑萍萍 余圣涛[3,4] 王黎明 Pingping Zheng;Shengtao Yu;Liming Wang(Henan Institutes of Advanced Technology,Zhengzhou University,Zhengzhou 450003,China;CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 100049,China;CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,National Center for Nanoscience and Technology of China,Beijing 100190,China;Sino-Danish College,University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]郑州大学河南先进技术研究院,郑州450003 [2]中国科学院高能物理研究所,中国科学院纳米生物效应与安全性重点实验室,北京100049 [3]国家纳米科学中心,中国科学院纳米生物效应与安全性重点实验室,北京100190 [4]中国科学院大学中丹学院,北京100049

出　　处：《中国科学：化学》2023年第11期2157-2174,共18页SCIENTIA SINICA Chimica

基　　金：国家重点研发计划(编号:2020YFA0710702,2021YFA1200900);国家自然科学基金(编号:31971322,32371469,32350410425);北京市自然科学基金(编号:IS23023);职业安全健康北京市重点实验室开放课题(编号:BKL-OSH-202201);中国科学院依托重大科技基础设施的建制化科研平台项目资助。

摘　　要：纳米材料与生物分子的相互作用直接影响着纳米材料的体内行为、状态与生物学效应,建立和发展可靠的分析方法以表征两者的相互作用非常必要.本文围绕纳米材料与蛋白质、脂类等重要生物分子作用过程的科学问题如作用分子类型的鉴定与相对丰度的测量、作用方式与界面结构,重点介绍基于同步辐射与散裂中子源等大科学装置的前沿分析方法在相关领域的应用,概述了相关分析方法的优势与先进性,为纳米生物效应研究、纳米医学应用的研究等提供了重要分析手段.最后,展望了新一代光源助力于纳米材料与生物分子作用研究的前景.Interaction between nanomaterials and biological molecules determines the behaviors and states of nanomaterials,and their biological responses and effects,which requires the development of suitable analytical methods and application in characterizing the interaction.Herein,we introduced a series of analytical methods for nanomaterialprotein/nanomaterial-lipid interaction and emphasized the techniques based on large-scale scientific facilities such as synchrotron radiation and spallation neutron source.We next showed how to use these advanced techniques in exploring the interaction details about the identity of binding molecules,the adsorption modes,and nanomaterial-biointerfacial structure and also indicated the advantages of these techniques compared with the conventional methods.These methods offer powerful analytical strategy during the study of biological effects of nanomaterials and the nanomedicine.In the end,we proposed the prospects about how the new generation of large-scale scientific facilities contributes to the study of nanomaterials and biomolecular interaction and biological effects.

关 键 词：同步辐射装置 散裂中子源 蛋白冠 纳米材料与脂质作用 纳米-生物界面 

分 类 号：G63[文化科学—教育学]