基于先进紫外光源的生物质谱技术的最新进展及展望  

Progress and prospects of biomolecule mass spectrometry based on advanced ultraviolet light source

作　　者：王方军[1,2] 刘哲益[1,2] 殷志斌 罗盼 杨诗蕊 肖春雷[2] 杨学明[2,3] Fangjun Wang;Zheyi Liu;Zhibin Yin;Pan Luo;Shirui Yang;Chunlei Xiao;Xueming Yang(CAS Key Laboratory of Separation Sciences for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;State Key Laboratory of Molecular Reaction Dynamics,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;Institute of Advanced Science Facilities,Shenzhen 518107,China)

机构地区：[1]中国科学院分离分析化学重点实验室,中国科学院大连化学物理研究所,大连116023 [2]大连光源科学研究室,分子反应动力学国家重点实验室,中国科学院大连化学物理研究所,大连116023 [3]深圳综合粒子设施研究院,深圳518107

出　　处：《中国科学：化学》2023年第11期2290-2303,共14页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金委科学中心项目(编号:22288201);中国科学院关键技术团队项目(编号:GJJSTD20220001)资助。

摘　　要：生物大分子质谱是研究复杂生命体系中分子的序列组成、动态结构、相互作用和空间分布的前沿科学领域.先进紫外光源特别是同步辐射、自由电子激光等大科学装置的发展,为光解离-串联质谱和光电离-质谱成像等生物大分子质谱研究尖端技术的进步提供了新的机遇.本综述中,我们总结了不同波长先进光源包括X射线、极紫外激光等在蛋白质、脂质等生物分子高效光解离和序列、结构、相互作用表征,光化学氧化标记和动态结构分析,高效光电离和高灵敏度质谱检测及空间分布成像等领域的研究进展;进一步展望了新一代先进紫外光源特别是具有更高亮度、更短脉冲、更高重频的极紫外自由电子激光在生物大分子质谱研究中的应用前景和可能带来的技术突破.Biomolecule mass spectrometry is an emerging scientific field for the exploration of sequence composition,dynamic structure,interaction,and spatial distribution of biomolecules in complex life systems.With the development of advanced ultraviolet light sources,especially large scientific facilities,such as synchrotron radiation and free electron laser,new opportunities have been provided for the progress of core technologies of cutting-edge biomolecule mass spectrometry such as photodissociation-tandem mass spectrometry and photoionization-mass spectrometry imaging,which has attracted extensive attention worldwide.In this review,we summarize the research progress of advanced ultraviolet light sources with different wavelengths,including X-ray and extreme ultraviolet lasers,in the fields of photodissociation for sequence and interaction characterization of biomolecules such as proteins and lipids,photochemical oxidation labeling for dynamic structural characterization,and efficient photoionization for highsensitive mass spectrometry detection and spatial distribution imaging.Further prospects are made for the application of the new generation of advanced light sources,especially extreme ultraviolet free electron laser with higher brightness,shorter pulse,and higher repetition rate,in the field of biomolecule mass spectrometry investigation.

关 键 词：先进紫外光源 生物大分子质谱 极紫外自由电子激光 蛋白质序列和结构表征 质谱成像 

分 类 号：G63[文化科学—教育学]