口腔黏膜上皮培养与眼表移植的研究进展  

Research progress on oral mucosal epithelial culture and ocular surface transplantation

作　　者：龚丹妮(综述) 严晨曦(审校)[1] 傅瑶(审校)[1] Gong Danni;Yan Chenxi;Fu Yao(Department of Ophthalmology,Shanghai Ninth People's Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology,Shanghai 200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院眼科、上海市眼眶病眼肿瘤重点实验室,上海200011

出　　处：《中华实验眼科杂志》2023年第11期1135-1139,共5页Chinese Journal Of Experimental Ophthalmology

基　　金：国家自然科学基金项目(81770888、82271041)。

摘　　要：人角膜上皮细胞由角膜缘干细胞不断补充,从而维持眼表稳态及正常视觉功能。热化学烧伤、Stevens-Johnson综合征等眼表损伤或疾病可导致角膜缘干细胞缺损,严重影响患者视力。当双眼角膜缘干细胞缺损时可考虑非角膜缘性自体上皮组织移植。口腔黏膜上皮细胞是眼表重建的重要种子细胞来源,体外培养构建的细胞片已在临床应用中取得良好效果。本文围绕载体、培养条件及技术等影响口腔黏膜上皮片培养的各种因素,以及细胞片应用于临床眼表移植的优缺点,对利用口腔黏膜上皮治疗角膜缘干细胞缺损的研究进展进行综述,以期为安全有效地重建眼表上皮提供研究方向。Human corneal epithelial cells are continuously replenished by limbal stem cells to maintain ocular homeostasis and normal visual function.Some ocular surface injuries or diseases,such as thermochemical burns and Stevens-Johnson syndrome,can result in limbal stem cell deficiency,severely compromising patients'visual acuity.Non-limbal autologous epithelial tissue transplantation should be considered when both eyes are affected.Oral mucosal epithelial cells are an important source of seed cells for ocular surface reconstruction.What's more,cell sheets constructed ex vivo have achieved good results in clinical applications.This article reviews the research progress on the use of oral mucosal epithelial cells for the treatment of limbal stem cell deficiency,focusing on various factors including carriers,culture conditions,and techniques that may affect the culture of oral mucosal epithelial sheets.In addition,the strengths and weaknesses of the application of cell sheets in clinical ocular surface transplantation are also discussed,so as to provide a research direction for the safe and effective reconstruction of ocular surface epithelium.

关 键 词：口腔黏膜上皮细胞 体外培养 角膜缘干细胞缺损 眼表重建 

分 类 号：R77[医药卫生—眼科]