超高效液相色谱-串联质谱法筛查芽苗类蔬菜中90种植物生长调节剂和抗生素  

Screening of Plant Growth Regulators and Antibiotics in Sprout Vegetables by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：曾雪芳 齐春艳 谢爱华 雷毅 ZENG Xue-Fang;QI Chun-Yan;XIE Ai-Hua;LEI Yi(Guangdong Institute of Food Inspection(Guangdong Inspection Center of Wine and Spirits),Guangzhou,Guangdong 510435,China)

机构地区：[1]广东省食品检验所(广东省酒类检测中心),广东广州510435

出　　处：《粮油食品科技》2023年第6期119-130,共12页Science and Technology of Cereals,Oils and Foods

基　　金：广东省市场监督管理局科技项目(2021ZS01)。

摘　　要：对色谱质谱条件、净化方式、提取溶剂、除水剂、净化剂组成和配比等样品前处理条件进行了优化,建立了超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)检测芽苗类蔬菜中11种植物生长调节剂和79种抗生素药物的高通量筛查方法。芽苗类蔬菜样品经1%甲酸乙腈-EDTA-Mcllvaine缓冲溶液提取、QuEChERS净化,采用ACQUITY UPLC HSS T3色谱柱分离、梯度洗脱,采用电喷雾正负离子模式同时采集的多反应监测模式,基质曲线外标法定量。结果表明,90种化合物的定量限在0.1~15.4μg/kg范围内,平均回收率范围为36.4%~137.3%,相对标准偏差范围均小于18.6%,实现了芽苗类蔬菜中90种高风险物质的同时、快速、高通量筛查和检测,有利于更充分地了解芽苗类蔬菜的质量安全问题。In this study,chromatographic and mass spectrometry conditions,sample cleanup and extraction,composition and ratio of salt and purifiers were optimized,which established a high-throughput screening method for 11 plant growth regulators and 79 antibiotic drugs in sprout vegetables by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Samples were extracted by 1% acetonitrile-EDTA-mcllvaine buffer solution,purified by QuEChERS,and separated by ACQUITY UPLC HSS T3 column with gradient elution.Electrospray positive and negative ion mode was used in multi-reaction monitoring mode,and matrix curve external standard method was used to quantify.The limits of quantitation of 90 compounds were in the range of 0.1~15.4 μg/kg.The average recoveries were in the range of 42.2%~138.6% with relative standard deviations lower than 17.2%.In this study,simultaneous,rapid and high-throughput screening and detection of 90 high-risk substances in sprout vegetables were established,which is conducive to a more full understanding of the quality and safety problems of sprout vegetables.

关 键 词：超高效液相色谱-串联质谱法 筛查 芽苗类蔬菜 植物生长调节剂 抗生素 

分 类 号：TS207.7[轻工技术与工程—食品科学]