类普鲁士蓝纳米酶材料的制备及其酶催化反应动力学探究——推荐一个分析化学综合实验  

Preparation of Prussian Blue Analogs Derived Nanoenzymes and Their Enzymatic Catalytic Reaction Kinetics:A Comprehensive Analytical Chemical Experiment

作　　者：刘梦婷 杨树芬 薛雨 臧嘉妍 苏权燕 郑兴烨 曹秋娥[1] 周川华 Mengting Liu;Shufen Yang;Yu Xue;Jiayan Zang;Quanyan Su;Xingye Zheng;Qiue Cao;Chuanhua Zhou(National Demonstration Center for Experimental Chemistry and Chemical Engineering Education(Yunnan University),School of Chemical and Technology,Yunnan University,Kunming 650091,China)

机构地区：[1]云南大学化学科学与工程学院,化学化工国家级实验教学示范中心(云南大学),昆明650091

出　　处：《大学化学》2023年第9期163-171,共9页University Chemistry

摘　　要：将类普鲁士蓝纳米酶引入本科实验教学,设计了一个探究性的分析化学综合实验,实验内容包括三种类普鲁士蓝纳米酶材料的制备、表征和酶催化反应速率常数的测定,并将这些纳米酶材料应用于过氧化氢(H2O2)的分析检测。本实验所用试剂价廉易得、反应条件温和、实验现象直观、时间分配合理,适合本科实验教学,用于提升学生的实验技能和科研创新能力。An exploratory analytical chemistry comprehensive experiment was designed based on Prussian blue analog-derived nanoenzymes.The experiment includes the preparation and characterization of three kinds of Prussian blue analog-derived nanoenzyme materials and the determination of their enzyme catalytic reaction rate constants.These nanomaterials were further applied to hydrogen peroxide detection.As this experiment features inexpensive reagents,mild reaction conditions,obvious experimental phenomena,and reasonable time allocation,it is suitable for undergraduate laboratory teaching to improve students’experimental skills and cultivate their scientific research and innovation ability.

关 键 词：类普鲁士蓝纳米材料 酶催化反应动力学 米氏常数 H_(2)O_(2)检测 紫外-可见分光光度法 

分 类 号：G64[文化科学—高等教育学] O6[文化科学—教育学]