基于TCGA数据库分析CD70在肝细胞癌中的表达及其对肿瘤微环境的影响  

Expression of CD70 in hepatocellular carcinoma and its impact on tumor microenvironment based on TCGA database

作　　者：龙志华[1] 徐青[1] Long Zhihua;Xu Qing(Department of General Surgery,Beijing Boai Hospital,China Rehabilitation Research Center,Beijing 100068,China)

机构地区：[1]中国康复研究中心、北京博爱医院普外科,北京100068

出　　处：《国际外科学杂志》2023年第10期676-681,I0005,I0006,共8页International Journal of Surgery

基　　金：中国康复研究中心重点课题(2019ZX-10)。

摘　　要：目的研究CD70及受体CD27在肝细胞癌(HCC)中的表达水平,及其与临床病理特征及预后的关系,并探讨CD70对肿瘤微环境可能的作用。方法下载癌症基因组图谱(TCGA)数据库中有关HCC的RNA测序数据,分析CD70和CD27在肿瘤组织和正常组织中的表达差异及其与临床病理特征的关系;采用Kaplan-Meier生存曲线比较CD70、CD27表达不同患者的生存率;分析CD70与其他影响肿瘤微环境基因表达间的相关性。结果通过TCGA数据库共获得371例HCC肿瘤样本和50例正常组织样本信息;CD70在HCC肿瘤组织内的表达水平显著高于正常组织;CD70表达水平与年龄、性别、肿瘤分期、是否存在远处转移及生存无明显相关性(P>0.05);CD27在HCC肿瘤组织内的表达水平与正常组织无显著差异,但CD27高表达组的总生存率显著高于CD27低表达组患者;HCC肿瘤组织内CD70表达与IL-12、FOXP3、CD25、CD39、CD27、PD-1、PD-L1、LAG3、CTLA4、Tim3、VEGF、IDO、CXCR4和CCL2的表达呈显著正相关(P<0.05,|r|>0.3)。结论CD70在HCC患者肿瘤组织中异常表达,虽然CD70表达升高与患者病理分期和生存无显著相关,但是其与免疫抑制肿瘤微环境相关,可能是HCC形成免疫逃逸的机制之一。Objective To investigate the expression level of CD70 and receptor CD27 in hepatocellular carcinoma(HCC)and their relationship with clinicopathological features and prognosis,and to explore the possible role of CD70 in the tumor microenvironment.Methods Download the RNA sequencing data related to HCC from The Cancer Genome Atlas(TCGA),and analyze the expression differences of CD70 and CD27 between HCC and normal tissues,and their relationship with clinicopathological features.Kaplan-Meier survival curve was used to compare the survival rate of patients with different expressions of CD70 and CD27.The correlation between CD70 and other genes that affect the expression of tumor microenvironment was analyzed.Results The data of 371 HCC tumor samples and 50 normal tissue samples were obtained by TCGA database.The expression of CD70 in HCC tumor tissues was significantly higher than that in normal tissues.There was no significant correlation between CD70 expression and age,gender,tumor stage,distant metastasis and survival(P>0.05).The expression of CD27 in HCC tumor tissues was not significantly different from that in normal tissues,but the overall survival rate of patients with high CD27 expression was significantly higher than that of patients with low CD27 expression.The expression of CD70 in HCC tumor tissue was significantly positively correlated with the expressions of IL-12,FOXP3,CD25,CD39,CD27,PD-1,PD-L1,LAG3,CTLA4,Tim3,VEGF,IDO,CXCR4 and CCL2(P<0.05,|r|>0.3).Conclusion CD70 is abnormally expressed in HCC.Although CD70 expression is not significantly associated with pathological staging and survival in HCC patients,it is related to the immunosuppressive tumor microenvironment,which may be one of the mechanisms of immune escape in HCC.

关 键 词：肝细胞癌 抗原 CD70 抗原 CD27 免疫检查点 肿瘤微环境 

分 类 号：R735.7[医药卫生—肿瘤]