人着丝粒蛋白E在人肾透明细胞癌中的表达及对细胞增殖的影响  

Expression of human centromere protein E in human renal clear cell carcinoma and its effects on the cell proliferation

作　　者：刘芬 李世宾 李晓石 LIU Fen;LI Shi-bin;LI Xiao-shi(Department of Pathology,Xinxiang Central Hospital,The Fourth Clinical College of Xinxiang Medical College,Xinxiang 453000,China;Department of Urology,Xinxiang Central Hospital,The Fourth Clinical College of Xinxiang Medical College,Xinxiang 453000,China)

机构地区：[1]新乡市中心医院,新乡医学院第四临床学院病理科,新乡453000 [2]新乡市中心医院,新乡医学院第四临床学院泌尿外科,新乡453000

出　　处：《天津医科大学学报》2023年第6期594-598,共5页Journal of Tianjin Medical University

摘　　要：目的:探讨人着丝粒蛋白E(CENPE)在人肾透明细胞癌组织中的表达、临床意义及对细胞增殖的影响。方法:生物信息学分析GEPIA数据库中CENPE在肾透明细胞癌组织及癌旁组织中的mRNA水平,并分析其表达与患者预后之间的关系。回顾性分析来自新乡市中心医院2014年9月—2021年8月接受手术治疗的77例肾透明细胞癌患者的临床病理资料。采用免疫组化法检测肾透明细胞癌组织和癌旁组织中CENPE蛋白表达水平,分析其与患者临床病理特征的关系。培养HTB-47和CRL-1932细胞,通过CENPE的shRNA质粒转染降低其表达,通过集落形成实验及CCK-8实验检测对肾透明细胞癌细胞增殖的影响。结果:生物信息学分析显示肾透明细胞癌患者CENPE显著高表达,并与无病生存期显著相关。免疫组化结果显示CENPE在肾透明细胞癌组织中显著高表达,而在癌旁组织中则为阴性或低表达,表达水平与肿瘤直径显著相关(P=0.021),而与患者的年龄、性别、肿瘤分化无关(均P>0.05)。集落形成实验(P=0.013 5、0.015 4)及CCK-8实验(P=0.009 1、0.013 3)证实CENPE在细胞表达下调并可以抑制肾透明细胞癌细胞的增殖。结论:肾透明细胞癌组织中CENPE显著高表达,且与患者临床病理特征及预后相关,CENPE蛋白促进肾透明细胞癌细胞的增殖。Objective:To investigate the expression and clinical significance of human centromere protein E(CENPE)in human renal clear cell carcinoma and its effect on cell proliferation.Methods:The mRNA levels of CENPE in renal clear cell carcinoma and paracarcinoma tissues were analyzed in GEPIA database,and the relationship between CENPE expression and prognosis of patients was analyzed.The clinicopathologic data of 77 patients with renal clear cell carcinoma who received surgical treatment from September 2014 to August 2021 at Xinxiang Central Hospital were retrospectively analyzed.Immunohistochemistry was used to detect the expression of CENPE protein in renal clear cell carcinoma and para-carcinoma tissues,and the relationship between CENPE protein expression and clinicopathologic features of patients was analyzed.HTB-47 and CRL-1932 cells were cultured and transfected with shRNA plasmid of CENPE to reduce the expression of HTB-47 and CRL-1932 cells.The effects on the proliferation of renal clear cell carcinoma cells were detected by colony formation assay and CCK-8 assay.Results:Bioinformatics analysis revealed that CENPE was significantly overexpressed in renal clear cell carcinoma patients and was significantly correlated with disease-free survival.Immunohistochemistry revealed significant high expression of CENPE in renal clear cell carcinoma tissues,while negative or low expression in para-carcinoma tissues.The expression level was significantly correlated with tumor size(P=0.021),but not with patient age,sex,or tumor differentiation(all P>0.05).Colony formation assay(P=0.0135,0.0154)and CCK-8 assay(P=0.0091,0.0133)confirmed that CENPEdown-regulationin cell expression inhibited the proliferation of renal clear cell carcinoma cells.Conclusion:CENPE is highly expressed in renal clear cell carcinoma,which is related to clinicopathological features and prognosis of patients.CENPE protein promotes the proliferation of renal clear cell carcinoma cells.

关 键 词：人着丝粒蛋白E 肾透明细胞癌 免疫组化 细胞增殖 

分 类 号：R737.1[医药卫生—肿瘤]