黄芩苷对注意缺陷多动障碍动物模型SHR大鼠核心症状影响研究  

Effect of baicalein on core symptoms in an SHR model of attention deficit hyperactivity disorder

作　　者：张永婷 周荣易 马丙祥[2] 吴晨蕾 谢新月 丁雪颖 ZHANG Yongting;ZHOU Rongyi;MA Bingxiang;WU Chenlei;XIE Xinyue;DING Xueying(Pediatric Medical College of Henan University of Traditional Chinese Medicine,Zhengzhou 450000,China;the First Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou 450000)

机构地区：[1]河南中医药大学儿科医学院,郑州450000 [2]河南中医药大学第一附属医院,郑州450000

出　　处：《中国比较医学杂志》2023年第11期25-32,149,共9页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金青年基金(82104928);中国博士后科学基金面上项目(2021M701123);河南省特色骨干学科课题(STG-ZYX06-202150);河南中医药大学仲景青年学者科研启动项目(CZ0168-04)。

摘　　要：目的通过旷场和Morris水迷宫实验,探究黄芩苷调控注意缺陷多动障碍动物模型核心临床症状的疗效及其潜在机制。方法将30只SHR大鼠随机分为模型组、盐酸哌甲酯(MPH)组、黄芩苷组、黄芩苷+丁苯那嗪组和MPH+丁苯那嗪组,每组6只,另设6只WKY大鼠为对照组。MPH组(1.5 mg/kg)、黄芩苷组(150 mg/kg)按体重(1 mL/100 g)灌胃给药,空白组、模型组给等量生理盐水,MPH+丁苯那嗪组、黄芩苷+丁苯那嗪组在灌胃给药同时,按体重(0.5 mL/100 g)腹腔注射丁苯那嗪(3 mg/kg)。连续给药4周。在规定时间进行旷场和水迷宫实验,记录并分析实结果。结果MPH组和黄芩苷组在旷场实验中运动总距离和平均速度较模型组显著减小(P<0.05),在水迷宫实验中潜伏期较模型组显著缩短(P<0.05)、在目标象限运动距离和停留时间占比及穿越平台次数较模型组显著增加(P<0.05),且这两组间无明显差异;黄芩苷+丁苯那嗪组在旷场实验中运动总距离和平均速度较模型组显著减小(P<0.05),较黄芩苷组明显增大,在水迷宫实验中潜伏期较模型组显著缩短(P<0.05),较黄芩苷组显著延长(P<0.05)、在目标象限运动距离和停留时间占比较模型组明显增加,较黄芩苷组显著减少(P<0.05)。结论黄芩苷能够改善SHR大鼠多动、冲动、注意力不集中等核心症状,其疗效的发挥可能与调控DA囊泡转运相关。ObjectiveTo explore the efficacy and potential mechanism of baicalin in regulating the core clinical symptoms of ADHD by Morris water maze and open field tests.MethodsThirty SHRs were randomly divided into five groups:model,methylphenidate hydrochloride(MPH),baicalin,baicalin+tetrabenazine,and MPH+tetrabenazine groups with six rats in each group.Another six WKY rats were used as the normal control group.Rats in the MPH group(1.5 mg/kg)and baicalin group(150 mg/kg)were administered the corresponding drugs(1 mL/100 g)by gavage,and those in normal control and model groups were administered an equal volume of normal saline by gavage.In addition to the corresponding drug gavage,rats in MPH+tetrabenazine and baicalin+tetrabenazine groups were subjected to intraperitoneal injection of tetrabenazine(3 mg/kg)in accordance with body weight(0.5 mL/100 g).The course was 4 weeks for all groups.Open field and Morris water maze experiments were conducted at predefined time points to record and analyze the result.ResultsIn the open field test,the total distance and average speed of rats in the MPH and baicalin groups were significantly lower than those in the model group(P<0.05).In the Morris water maze test,the latency of rats in the MPH and baicalin groups was significantly shorter than that of the model group(P<0.05),and the proportion of movement distance and residence time in the target quadrant and the number of times crossing the platform in the MPH and baicalin groups were significantly higher than those in the model group(P<0.05),and there is no significant difference between the two grups.The total distance and average speed in the baicalin+tetrabenazine group were significantly lower than those in the model group(P<0.05)and larger than those in baicalin group in the open field test.The latency in the baicalin+tetrabenazine grup was significantly shorter than that in the model group(P<0.05)and was significantly longer than that in baicalin group(P<0.05)in the Morris water maze test.The movement distance and residence t

关 键 词：注意缺陷多动障碍 自发性高血压大鼠 黄芩苷 行为学实验 核心症状 

分 类 号：R-33[医药卫生]