Core–Shell Microfiber Encapsulation Enables Glycerol‑Free Cryopreservation of RBCs with High Hematocrit  

作　　者：Xianhui Qin Zhongrong Chen Lingxiao Shen Huilan Liu Xilin Ouyang Gang Zhao 

机构地区：[1]Department of Electronic Engineering and Information Science,University of Science and Technology of China,Hefei 230027,People’s Republic of China [2]School of Biomedical Engineering,Anhui Medical University,Hefei 230022,People’s Republic of China [3]Department of Blood Transfusion,The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230001,People’s Republic of China [4]The Fourth Medical Center,Chinese PLA General Hospital,Beijing 100089,People’s Republic of China

出　　处：《Nano-Micro Letters》2024年第1期35-54,共20页纳微快报（英文版）

基　　金：the National Natural Science Foundation of China(No.82172114);the Anhui Provincial Natural Science Foundation for Distinguished Young Scholars(No.2108085J37).

摘　　要：Cryopreservation of red blood cells(RBCs)provides great potential benefits for providing transfusion timely in emergencies.High concentrations of glycerol(20%or 40%)are used for RBC cryopreservation in current clinical practice,which results in cytotoxicity and osmotic injuries that must be carefully controlled.However,existing studies on the low-glycerol cryopreservation of RBCs still suffer from the bottleneck of low hematocrit levels,which require relatively large storage space and an extra concentration process before transfusion,making it inconvenient(time-consuming,and also may cause injury and sample lose)for clinical applications.To this end,we develop a novel method for the glycerol-free cryopreservation of human RBCs with a high final hematocrit by using trehalose as the sole cryoprotectant to dehydrate RBCs and using core–shell alginate hydrogel microfibers to enhance heat transfer during cryopreservation.Different from previous studies,we achieve the cryopreservation of human RBCs at high hematocrit(>40%)with high recovery(up to 95%).Additionally,the washed RBCs post-cryopreserved are proved to maintain their morphology,mechanics,and functional properties.This may provide a nontoxic,high-efficiency,and glycerol-free approach for RBC cryopreservation,along with potential clinical transfusion benefits.

关 键 词：CRYOPRESERVATION Human red blood cells Core-shell microfibers TREHALOSE HEMATOCRIT 

分 类 号：R73[医药卫生—肿瘤]