核糖体调控因子1对人乳腺癌MDA-MB-468细胞增殖和转移能力的影响  

Effect of regulator of ribosome synthesis 1 on proliferation and metastasis abilities of human breast cancer MDA-MB-468 cells

作　　者：王润泽 彭翠修 宋军莹 侯琳[1] WANG Runze;PENG Cuixiu;SONG Junying;HOU Lin(Department of Biochemistry and Molecular Biology,School of Basic Medicine,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院生物化学与分子生物学系,山东青岛266071

出　　处：《精准医学杂志》2024年第1期5-10,共6页Journal of Precision Medicine

基　　金：国家自然科学基金资助项目(81472542);山东省重点研发项目(2019GSF107025)。

摘　　要：目的探讨核糖体合成调控因子1(RRS1)对人乳腺癌细胞增殖和转移能力的影响。方法通过Western Blot实验检测人乳腺癌细胞系(MDA-MB-231、MDA-MB-468、BT549、MCF-7细胞)以及正常人乳腺上皮细胞中RRS1蛋白的表达量;将MDA-MB-468细胞分别感染sh-RRS1慢病毒(sh-RRS1组)和阴性对照慢病毒(Con组),未进行任何感染的MDA-MB-468细胞为Blank组,在荧光显微镜下观察Con组和sh-RRS1组慢病毒感染效率,采用实时荧光定量PCR(RT-qPCR)技术和Western Blot实验分别检测各组细胞中RRS 1 mRNA和蛋白的表达水平;采用CCK-8实验检测RRS1对MDA-MB-468细胞活力的影响;采用划痕实验、Transwell实验以及侵袭实验检测RRS1对MDA-MB-468细胞侵袭和迁移能力的影响。结果各乳腺癌细胞系中RRS1的表达水平均明显高于正常人乳腺上皮细胞(F=28.71,P<0.05);相较于Blank组和Con组,sh-RRS1组细胞中RRS 1 mRNA和蛋白的表达水平均显著降低(F=118.10、335.40,P<0.05),细胞增殖活力明显减弱(F=825.60~2839.00,P<0.05),侵袭和迁移能力明显降低(F=25.60~430.80,P<0.05)。结论RRS 1基因可能参与了乳腺癌细胞的增殖和转移过程,其作用可能与细胞中RRS 1的高表达有关。Objective To investigate the effect of regulator of ribosome synthesis 1(RRS1)on the proliferation and metastasis abilities of human breast cancer cells.Methods Western Blot was used to measure the protein expression level of RRS1 in human breast cancer cell lines(MDA-MB-231,MDA-MB-468,BT549,and MCF-7)and normal human breast epithelial cells.MDA-MB-468 cells were infected with sh-RRS1 lentivirus(sh-RRS1 group)and negative-control lentivirus(Con group),and MDA-MB-468 cells without infection were established as Blank group.Lentiviral infection efficiency was observed under a fluorescence microscope for the Con group and the sh-RRS1 group,and quantitative real-time PCR and Western blot were used to measure the mRNA and protein expression levels of RRS1 in cells;CCK-8 assay was used to observe the effect of RRS1 on the viability of MDA-MB-468 cells;scratch assay,Transwell assay,and invasion assay were used to observe the effect of RRS1 on the invasion and migration abilities of MDA-MB-468 cells.Results The expression level of RRS1 in the breast cancer cell lines was significantly higher than that in normal human breast epithelial cells(F=28.71,P<0.05).Compared with the Blank group and the Con group,the sh-RRS1 group had significant reductions in the mRNA and protein expression levels of RRS1(F=118.10,335.40,P<0.05),cell proliferation activity(F=825.60-2839.00,P<0.05),and the invasion and migration abilities of cells(F=25.60-430.80,P<0.05).Conclusion The RRS 1 gene might be involved in the proliferation and metastasis of breast cancer cells,which may be associated with the high expression of RRS 1 in cells.

关 键 词：乳腺肿瘤 细胞系 肿瘤 核蛋白质类 核糖体调控因子1 细胞增殖 细胞运动 

分 类 号：R737.9[医药卫生—肿瘤]