SIRT1基因启动子区rs3758391位点突变与不同分级老年高血压易感性及疗效关系  

Study on the relationship between the rs3758391 mutation in the promoter region of SIRT1 gene and susceptibility and efficacy of different grades of elderly hypertension

作　　者：徐炜棉 杨凯瑞 李宁 王国峰 XU Wei-mian;YANG Kai-rui;LI Ning;WANG Guo-feng(The Second Department of Cadre Ward,The 960th Hospital of the PLA,Jinan Shandong 250031,China)

机构地区：[1]解放军第九六〇医院干部病房二科,山东济南250031

出　　处：《蚌埠医学院学报》2023年第11期1537-1540,共4页Journal of Bengbu Medical College

摘　　要：目的:探讨沉默信息调节因子1(SIRT1)基因启动子区rs3758391位点突变与不同分级老年高血压易感性的关系以及基因型与应用硝苯地平治疗效果的关系。方法:选取老年高血压病人220例作为观察组,同期健康体检者185名作为对照组。采用TaqMan法检测SIRT1基因rs3758391位点多态性,分析SIRT1基因多态性与老年原发性高血压的关系。观察组病人接受硝苯地平治疗,比较不同基因型病人治疗前后舒张压(DBP)、收缩压(SBP),分析基因型对疗效的预测价值。结果:SIRT1基因rs3758391位点存在T、C 2种等位基因,观察组和对照组基因型和等位基因频率分布差异均有统计学意义(P<0.01),观察组不同高血压分级病人基因型和等位基因频率分布差异均有统计学意义(P<0.01)。观察组1~3级高血压病人经硝苯地平治疗后,SBP、DBP均较治疗前明显降低(P<0.01),且TT基因型病人治疗后SBP和DBP均低于CT、CC基因型(P<0.05~P<0.01)。220例病人中控制不良者34例,控制良好者186例,TT型病人的控制良好率高于CT+CC型病人(P<0.05)。结论:SIRT1基因启动子区rs3758391位点突变与不同分级老年高血压易感性以及血压控制效果密切相关,其中含有C等位基因的病人高血压分级较高,血压控制效果较差,可考虑纳入SIRT1作为高血压疗效评估标志物。Objective:To investigate the relationship between the mutation of rs3758391 in the promoter region of the silent information regulator 1(SIRT1)gene and susceptibility of different grades of elderly hypertension,and relationship between genotype and therapeutic effects of nifedipine.Methods:Two hundred and twenty elderly hypertensive patients and 185 healthy subjects were divided into the observation group and control group,respectively.The rs3758391 polymorphism of SIRT1 gene was detected using the TaqMan method,and the relationship between SIRT1 gene polymorphism and senile essential hypertension was analyzed.The observation group was treated with nifedipine.The levels of SBP and DBP in patients with different genotypes were compared before and after treatment,and the predictive value of genotypes for curative effect was analyzed.Results:There were two alleles of T and C at the rs3758391 locus of the SIRT1 gene.The differences of the genotype and allele frequency distribution between two groups were statistically significant(P<0.01),and the differences of the genotype and allele frequency distribution in different hypertension grades patients of observaion group were statistically significant(P<0.01).After nifedipine treatment,the SBP and DBP of patients with grade 1-3 hypertension in the observation group significantly decreased compared with before treatment(P<0.01),and those in TT genotype patients were lower than which in CT and CC genotype patients(P<0.05 to P<0.01).Among 220 patients,34 cases were poorly controlled and 186 cass were well controlled.The good control rate of TT patients was higher than that of CT+CC patients(P<0.05).Conclusions:The rs3758391 mutation in the promoter region of SIRT1 gene is closely related to the susceptibility of the elderly hypertension in different grades and blood pressure control effect.The patients with C allele have higher hypertension grade and poor blood pressure control effect,so the SIRT1 can be considered as a marker to evaluate the efficacy of hypertension.

关 键 词：高血压 沉默信息调节因子1 位点突变 易感性 

分 类 号：R544.1[医药卫生—心血管疾病]