Pan-cancer analysis of the prognostic and immunological role of nucleophosmin/nucleoplasmin 3(NPM3)and its potential significance in lung adenocarcinoma  

作　　者：Qianhui Wei Jing Zhou Xinyue Wang Zhaona Li Xiuqiong Chen Kaidi Chen Richeng Jiang 

机构地区：[1]Tianjin Medical University Cancer Institute&Hospital,National Clinical Research Center for Cancer,Tianjin 300202,China [2]Key Laboratory of Cancer Prevention and Therapy,Tianjin 300202,China [3]Tianjin's Clinical Research Center for Cancer,Tianjin 300202,China [4]Department of Thoracic Oncology,Tianjin Lung Cancer Center,Tianjin Cancer Institute&Hospital,Tianjin Medical University,Tianjin 300202,China

出　　处：《Cancer Pathogenesis and Therapy》2023年第4期238-252,共15页癌症发生与治疗（英文）

基　　金：This study was supported by the National Natural Science Foundation of China(No.82172620);the Nature Science Foundation of Tianjin City(No.18JCZDJC98800).

摘　　要：Background Nucleophosmin/nucleoplasmin 3(NPM3),a member of the NPM protein family,is widely expressed in various human tissues.Although previous studies identified elevated NPM3 expression in several cancers,a systematic pan-cancer analysis remains lacking.In this study,we conducted a comprehensive analysis of NPM3 to determine its role in tumorigenesis and tumor development.Methods Using data from The Cancer Genome Atlas(TCGA)and various bioinformatics analysis tools,we conducted a pan-cancer analysis of NPM3.Additionally,we collected gene expression and clinical data from 890 patients with lung adenocarcinoma(LUAD)from TCGA and the Gene Expression Omnibus database.We performed Cox regression analyses to explore the independent prognostic value of NPM3 expression in LUAD and plotted a nomogram to predict patient survival.We also used real-time quantitative polymerase chain reaction(RT-qPCR)to examine the expression levels of NPM3 in seven pairs of LUAD and paraneoplastic tissue samples.Results NPM3 expression was significantly increased in 20 types of cancer and was associated with poor prognosis in five types(P<0.05).NPM3 expression was negatively correlated with DNA methylation and positively correlated with copy number variation.NPM3 was also significantly associated with immune cell infiltration in various cancers.Cox regression analyses revealed that NPM3 expression could serve as an independent prognostic marker of LUAD.Moreover,our nomogram demonstrated good predictive ability for the prognosis of patients with LUAD.Finally,the high expression of NPM3 in LUAD was verified using RT-qPCR.Conclusion NPM3 is a promising biomarker for predicting pan-cancer prognosis and immunotherapeutic efficacy.

关 键 词：NPM3 Pan-cancerLung adenocarcinoma Prognosis Immune infiltration Biomarker 

分 类 号：R734.2[医药卫生—肿瘤]