Host gut-derived Bacillus probiotics supplementation improves growth performance, serum and liver immunity, gut health, and resistive capacity against Vibrio harveyi infection in hybrid grouper(♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)  

作　　者：Kwaku Amoah Beiping Tan Shuang Zhang Shuyan Chi Qihui Yang Hongyu Liu Yuanzhi Yang Haitao Zhang Xiaohui Dong 

机构地区：[1]Laboratory of Aquatic Animal Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Zhanjiang,Guangdong 524088,China [2]Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province,Zhanjiang,Guangdong 524088,China [3]Key Laboratory of Aquatic,Livestock and Poultry Feed Science and Technology in South China,Ministry of Agriculture,Zhanjiang,Guangdong 524000,China

出　　处：《Animal Nutrition》2023年第3期163-184,共22页动物营养（英文版）

基　　金：supported financially by the General Program of Natural Science Foundation of Guangdong Province(2021A1515011165);the Department of Education of Guangdong Province (2021ZDZX4005);the National Natural Science Foundation of China (31972808);the Research and Demonstration of Precision Functional Compound Feed Technology of Major Cultured Fishes and Shrimps in South China (2021B0202050002);the China Agriculture Research System of MOF and MARA (CARS-47)。

摘　　要：Several reports have revealed the vital role that probiotics play in fish growth and health.However,few works are available for host gut-derived probiotics on the growth,immunity,and gut microbiota of fish,especially in hybrid grouper (♀Epinephelus fuscoguttatus×♂Epinephelus lanceolatus) due to their isolation difficulty and functional verification.This study aimed at assessing 3 host gut-derived Bacillus species?effects on the growth,immune and antioxidant-biochemical responses,haematological parameters,intestinal morphology,immune-related gene expression,gut microbiota,and disease resistance against Vibrio harveyi in hybrid grouper.A total of 480 hybrid grouper (initial weight=9.03±0.02 g) were randomly allotted into 4 groups,namely,the group fed a basal diet without probiotic inclusion (control,B0),the group fed the basal diet with Bacillus velezensis GPSAK4 (BV),the group fed the basal diet with Bacillus subtilis GPSAK9 (BS),and the group fed the basal diet with Bacillus tequilensis GPSAK2 (BT) strains at 1.0×10^(9)CFU/g.After a 6-week feeding trial,the results revealed significant improvements (P<0.05) in the growth performance,whole fish-body proximate composition,blood haematological parameters,serum,liver,and intestinal biochemical indexes,intestinal morphology,and protection against V.harveyi pathogen in the probiotic-treated groups compared with the untreated.Additionally,the expressions of intestinal tight junction genes (occludin and ZO1),pro-and anti-inflammatory genes,including IL1β,IL6,IL8,TNFa,MyD88,IL10,and TGFβ,were upregulated (P<0.05) after Bacillus species administration.Host gut-derived Bacillus supplementation shaped the gut microbiota by significantly increasing (P<0.05) the relative abundance of Proteobacteria,Bacteroidetes,Actinobacteria (except the BS group),Acidobacteria(except the BT group),Cyanobacteria (except the BV and BT groups),and Verrucomicrobia phyla,as well as known beneficial genera (Romboutsia,Turicibacter,Epulopiscium,Clostridium_sensu_stricto 1 and 13,Lactobacil

关 键 词：Hybrid grouper(♀Epinephelus fuscoguttatus×♂E.lanceolatus) Host-associated probiotics Immune and antioxidant-biochemical responses Gut microbiota Immune-related gene expression Vibrio harveyi 

分 类 号：R57[医药卫生—消化系统]