Effects of L-arginine,guanidinoacetic acid and L-citrulline supplementation in reduced-protein diets on bone morphology and mineralization of laying hens  

作　　者：Hiep Thi Dao Amy F.Moss Emma J.Bradbury Robert A.Swick 

机构地区：[1]School of Environmental and Rural Science,Faculty of Science,Agriculture,Business and Law,University of New England,Armidale,New South Wales,2351,Australia [2]Faculty of Animal Science,Vietnam National University of Agriculture,Trau Quy Town,Gia Lam District,Hanoi,100000,Vietnam [3]Baiada Poultry Pty Limited,Pendle Hill,New South Wales,2145,Australia

出　　处：《Animal Nutrition》2023年第3期225-234,共10页动物营养（英文版）

基　　金：Australian Egg Corporation Limited for their financial support for this study(grant number:1PS802)。

摘　　要：The alterations in feed ingredients and the nutrient matrix to produce reduced-protein diets may affect bone morphology and mineralization in laying hens.This study was implemented to determine the effects of L-arginine(Arg),guanidinoacetic acid(GAA),and L-citrulline(Cit)supplementation to Argdeficient reduced-protein diets on bone morphology,strength,and mineralization status of laying hens.Individually housed Hy-Line Brown laying hens were evenly distributed to five dietary treatments with 25 replicates per treatment from 20 to 40 wk of age.Treatments consisted of a standard protein diet(17%crude protein,SP),a reduced-protein diet deficient in Arg(13%crude protein,RP),and RP supplemented with Arg(0.35%Arg,RP-Arg),GAA(0.46%GAA equivalent to 0.35%Arg,RP-GAA),or Cit(0.35%Cit equivalent to 0.35%Arg,RP-Cit)to reach the Arg level of SP diets.Birds fed the SP diet had similar bone weight,ash,length,width,Seedor index,breaking strength,and serum mineral concentration,but higher toe B level(P<0.001)compared to those fed the RP diet at wk 40.Birds fed the SP diet consumed more but also excreted more K and B compared to those fed the RP diet(P<0.01).Birds fed the SP diet had lower Cu digestibility(P=0.01)and higher B retention(P<0.01)compared to those offered the RP diet.Supplementation of Arg,GAA,and Cit to the RP diet increased relative femur weight and length(P<0.001).Citrulline supplementation also increased relative tibia and femur ash,and Zn digestibility(P<0.05).Supplementation of GAA to the RP diet decreased serum Ca,P,and Mg levels,decreased tibia Fe and Mg levels and toe Mg level,but increased Al,Fe,Zn,and Mn digestibility(P<0.05).The current findings demonstrated the capacity of laying hens to adapt to low mineral intake by increasing mineral utilization.Overall,bone morphology and breaking strength,and serum mineral level in laying hens were not influenced by dietary CP levels.Dietary Arg,GAA,or Cit supplementation were effective in improving bone morphology and mineralization in laying hens fed Arg-deficient

关 键 词：Ash Breaking strength FEMUR Low protein Minerals DIGESTIBILITY 

分 类 号：R73[医药卫生—肿瘤]