荷叶碱改善高脂饮食诱导比格犬肥胖的试验研究  

Experimental study on the improvement of obesity induced by high-fat diet with nuciferine in beagles

作　　者：卢炜 刘超 卢劲晔[2] 陆江[2] LU Wei;LIU Chao;LU Jinye;LU Jiang(Department of Veterinary Medicine,Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China;Department of Pet Science and Technology,Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China)

机构地区：[1]江苏农牧科技职业学院动物医学院,江苏泰州225300 [2]江苏农牧科技职业学院宠物科技学院,江苏泰州225300

出　　处：《畜牧与兽医》2023年第12期122-128,共7页Animal Husbandry & Veterinary Medicine

基　　金：国家职业教育教师教学创新团队建设项目(教职成函[2021]7号);江苏农牧科技职业学院校级课题(NSF2021ZR08)。

摘　　要：本研究旨在评估荷叶碱(NUC)对高脂饮食比格犬的抗肥胖效果和安全性。试验选取36只成年比格犬,适应性饲喂2周后随机等分为6组:正常饲粮组、含0.1%NUC正常饲粮组、高脂饲粮组、含0.05%NUC高脂饲粮组、含0.1%NUC高脂饲粮组和含0.15%NUC高脂饲粮组。饲喂8周后,进行血常规、血液生化和血液电解质检测,并对腹股沟皮下脂肪组织进行HE染色观察脂肪细胞大小。结果显示:高脂饲粮组的终末体重、体重增量、皮下脂肪厚度、脂肪细胞面积,血中甘油三酯(TG)、总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)等均高于正常饲粮组,添加0.1%NUC和0.15%NUC均可逆转这些变化。此外,添加0.1%NUC降低了高脂饮食犬总胆红素(TBIL)和丙氨酸氨基转移酶(ALT)水平,添加不同剂量NUC对血常规参数和血清电解质无影响。综上,NUC可改善高脂饮食诱导比格犬的肥胖和肝酶变化,0.1%的添加剂量效果最佳。This study was to evaluate the anti-obesity effect and safety of nuciferine(NUC) on high-fat diet beagles.After 2 weeks of adaptive feeding,a total of 36 adult beagles were used and randomly divided into 6 groups fed with normal diet,normal diet with 0.1% NUC,high fat diet with 0.05% NUC,high fat diet with 0.1% NUC,and high fat diet with 0.15% NUC,respectively.After 8 weeks of feeding,the tests of corpuscle,biochemistry and electrolyte in blood samples collected from the tested dogs were performed,and the subcutaneous adipose tissue of the groin was stained with H & E to observe the size of adipocytes.The results showed that the final body weight,body weight gain,subcutaneous fat thickness,adipocyte area,triglycerides(TG),total cholesterol(TC),and low-density lipoprotein cholesterol(LDL-C) in the high fat diet group were higher than those in the normal diet group,while supplementing 0.1% NUC and 0.15% NUC reversed these changes.In addition,supplementation of 0.1% NUC reduced the levels of total bilirubin(TBIL) and alanine aminotransferase(ALT) in the high-fat diet dogs.Supplementation of different doses of NUC had no effect on corpuscle and serum electrolytes in the blood of the animals.In summary,NUC in the diet improved the obesity and liver enzymes induced by a high-fat diet in beagles,with a 0.1% added dose having the best effect.

关 键 词：荷叶碱 比格犬 高脂饮食 肥胖 脂肪细胞 

分 类 号：S858[农业科学—临床兽医学]