昆布多糖WLP5对MCF-7乳腺癌荷瘤裸鼠肿瘤的抑制作用  

Antitumor Effect of Laminaria japonica Polysaccharide WLP5 in MCF-7 Breast Cancer Cell-Bearing Mice

作　　者：曹明原 李莹 綦国红 杨志萍 黄德春 陈贵堂 CAO Mingyuan;LI Ying;QI Guohong;YANG Zhiping;HUANG Dechun;CHEN Guitang(National R&D Center for Chinese Herbal Medicine Processing,College of Engineering,China Pharmaceutical University,Nanjing 211198,China)

机构地区：[1]中国药科大学工学院,国家中药材加工技术研发专业中心,江苏南京211198

出　　处：《食品科学》2023年第21期137-143,共7页Food Science

基　　金：“十三五”国家重点研发计划重点专项(2017YFD0400203)。

摘　　要：为探讨昆布多糖WLP5对MCF-7乳腺癌裸鼠免疫功能及肿瘤生长的影响,将MCF-7乳腺癌裸鼠随机分为模型组、阳性对照组(盐酸阿霉素,0.4μg/kg m_(b))、WLP5低剂量组(50μg/kg m_(b))和WLP5高剂量组(200μg/kg m_(b)),每组10只,隔天灌胃给药,共50 d。测定各实验组裸鼠肿瘤长径及短径、肿瘤体积、抑瘤率、血液学指标及白细胞介素-2(interleukin-2,IL-2)、IL-6、转化生长因子-β(transforming growth factor-β,TGF-β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)4种细胞因子的质量浓度,并观察各实验组裸鼠肝脏组织及肿瘤组织的病理切片。结果表明:与模型组相比,WLP5低剂量组与高剂量组裸鼠的肿瘤长径、短径及肿瘤体积均显著或极显著减小(P<0.05,P<0.01),其抑瘤率分别为36.5%和48.9%;WLP5高剂量组的单核细胞比率显著降低(P<0.05),平均血小板体积显著增加(P<0.05),其余各项血液指标均无明显变化;低、高剂量WLP5显著或极显著增加了细胞因子IL-2及IL-6的释放量(P<0.05,P<0.01);病理切片结果显示WLP5高剂量组裸鼠肝脏组织完整,而肿瘤细胞出现大面积坏死,细胞核消失,表明WLP5有较强的体内抗肿瘤作用,且对正常细胞或组织无明显毒性,其抗肿瘤作用与调节免疫功能有关。In order to investigate the effect of Laminaria japonica polysaccharide WLP5,obtained by hot water extraction,on immune function and the growth of tumor in MCF-7 breast cancer cell-bearing mice,MCF-7 tumor-bearing nude mice were randomly divided into four groups(n=10 each):model,positive control(doxorubicin hydrochloride,0.4μg/kg m_(b)),low-dose(50μg/kg m_(b))and high-dose(200μg/kg m_(b))WLP5.All mice were treated by intragastric administration every other day for 50 days.The long and short diameters of tumor,tumor volume,tumor inhibition rate,hematological indicators and the concentrations of serum cytokines including interleukin-2(IL-2),IL-6,transforming growth factor-β(TGF-β)and tumor necrosis factor-α(TNF-α)were determined and pathological changes of liver and tumor sections from mice in each group were observed.The results showed that the long diameter,short diameter and volume of tumor in the low-and high-dose WLP5 groups significantly decreased in comparison to the model group(P<0.05 or P<0.01),and the tumor inhibition rates were 36.5%and 48.9%,respectively.Compared with the model group,monocyte percentage significantly decreased(P<0.05)and mean platelet volume significantly increased in the high-dose group(P<0.05),but no significant differences were found in any other hematological indicators.Moreover,WLP5 at low and high doses significantly increased the release of IL-2 and IL-6(P<0.05 or P<0.01).The results of pathological sections suggested that the liver tissue of mice in the high-dose group was complete,while the tumor cells showed large areas of necrosis and the nuclei disappeared,indicating that WLP5 has strong antitumor effect in vivo without obvious toxicity to normal cells or tissues.The antitumor activity of WLP5 is related to the regulation of immune function.

关 键 词：昆布多糖 乳腺癌 免疫调节 体内抗肿瘤活性 细胞因子 

分 类 号：R285.5[医药卫生—中药学] TS201.4[医药卫生—中医学]