外源葡萄糖通过调控蔗糖代谢增强采后杏果实的抗冷性  

Exogenous Glucose Enhances Cold Resistance in Postharvest Apricot Fruit by Regulating Sugar Metabolism

作　　者：张昱 芦玉佳 赵亚婷 梁可欣 任新雅 刘志旭 石慧敏 朱璇 ZHANG Yu;LU Yujia;ZHAO Yating;LIANG Kexin;REN Xinya;LIU Zhixu;SHI Huimin;ZHU Xuan(College of Food Science and Pharmacy,Xinjiang Agricultural University,Ürümqi 830052,China)

机构地区：[1]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052

出　　处：《食品科学》2023年第21期167-174,共8页Food Science

基　　金：国家自然科学基金地区科学基金项目(31860462);南京农业大学-新疆农业大学联合基金项目(KYYJ201908)。

摘　　要：为探究外源葡萄糖处理对杏果实采后抗冷性与糖代谢的影响,本实验以新疆‘赛买提’杏为原料,采用不同浓度葡萄糖进行减压渗透处理,晾干后,置于温度(0±1)℃的冷库中贮藏49 d,定期测定杏果实冷害发病率、冷害指数、品质及糖代谢相关指标。结果表明:200 mmol/L外源葡萄糖处理可有效抑制杏果实冷害发病率、冷害指数与细胞膜渗透率的上升与丙二醛(malondialdehyde,MDA)的积累,较好地维持杏果实硬度、色泽、可溶性固形物质量分数(soluble solids content,SSC)、可滴定酸(titratable acidity,TA)质量分数与抗坏血酸(ascorbic acid,ASA)含量。同时,与对照组相比,200 mmol/L外源葡萄糖处理提高了杏果实酸性转化酶(acid invertase,AI)、中性转化酶(neutral invertase,NI)、蔗糖磷酸合成酶(sucrose phosphate synthase,SPS)和蔗糖合成酶(sucrose synthase,SS)活力,显著促进了贮藏过程中葡萄糖及果糖的积累,但在贮藏中后期(21~49 d)降低了蔗糖的含量。综上,外源葡萄糖处理可通过调控糖代谢相关酶的活性来增强葡萄糖与果糖的含量,从而增强杏果实抗冷性,维持果实贮藏品质。本实验结果可为控制杏果实采后冷害提供参考。This study was conducted in order to explore the effect of exogenous glucose treatment on postharvest cold resistance and sugar metabolism in apricot fruit.Xinjiang-grown‘Saimati’apricots were subjected to vacuum osmosis treatment with different concentrations of glucose followed by cold storage at(0±1)℃for 49 days.The incidence of chilling injury,chilling injury index,quality and sugar metabolism-related indicators of apricot fruit were measured at regular intervals during the storage period.The results showed that 200 mmol/L exogenous glucose treatment could effectively inhibit the increase in the incidence of chilling injury,chilling injury index,cell membrane permeability and the accumulation of malondialdehyde(MDA),and well maintain the hardness,color,soluble solids content(SSC),titratable acid(TA)and ascorbic acid(ASA)contents of apricot fruit.Meanwhile,compared with the control group,the exogenous glucose treatment increased the activities of acid invertase(AI),neutral invertase(NI),sucrose phosphate synthase(SPS)and sucrose synthase(SS)in apricot fruit,and significantly increased the accumulation of glucose and fructose during storage.However,the content of sucrose decreased during the mid-to-late stage of storage(21–49 days).In summary,exogenous glucose treatment could enhance the contents of glucose and fructose by regulating the activity of enzymes related to sugar metabolism,thereby enhancing cold resistance and maintaining the storage quality of the fruit.The results of this study can provide a reference for controlling chilling injury in postharvest apricot fruit.

关 键 词：杏果实 葡萄糖 冷害 果糖 糖代谢 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]