柑橘酸腐病菌PacC的生物信息学分析及其响应pH值的表达模式  

Bioinformatics Analysis of PacC Gene of Geotrichum citri-aurantii Isolated from Citrus Fruit and Its Expression Pattern in Response to pH

作　　者：郑杰 谭小丽 胡近近 陶能国[1] 欧阳秋丽 李茂慧 李路 ZHENG Jie;TAN Xiaoli;HU Jinjin;TAO Nengguo;OUYANG Qiuli;LI Maohui;LI Lu(College of Chemical Engineering,Xiangtan University,Xiangtan 411105,China)

机构地区：[1]湘潭大学化工学院,湖南湘潭411105

出　　处：《食品科学》2023年第22期108-115,共8页Food Science

基　　金：国家自然科学基金青年科学基金项目(32202140);湖南省自然科学基金项目(2021JJ40533);湖南省大学生创新创业训练计划项目(S202210530067)。

摘　　要：酸腐病菌(Geotrichum citri-aurantii)可调节侵染位点的pH值增强其致病性,pH值信号转导途径关键转录因子PacC在真菌响应环境pH值变化中发挥重要功能,但PacC在酸腐病菌中的作用尚不清楚。从酸腐病菌基因组中注释到2个PacC候选基因,对其进行生物信息学分析,并研究不同pH值条件下的表达模式。结果表明,PacC1与PacC2属于C2H2蛋白家族成员,且与指状青霉(Penicillium digitatum)PacC的亲缘关系较近。PacC1与PacC2在染色体位置、蛋白质分子质量和等电点等方面存在差异。离体条件下,酸腐病菌具有广泛的pH值适应性,最适pH值为3.0。PacC1和PacC2的表达均受pH值调控,且两个基因的表达趋势具有一定相似性。活体条件下,pH>3.0的宫川蜜橘果实组织中,酸腐病发病部位pH值随着发病进程显著下降,而pH<3.0的尤力克柠檬果实组织中,发病部位pH值显著上升,同时PacC1与PacC2的相对表达量均呈上升趋势。综上可知,环境pH值对酸腐病菌的生长有一定影响,酸腐病菌也对环境pH值有一定调节能力。由于基因结构和理化性质的差异,酸腐病菌的2个PacC对pH值的响应程度和方式有一定差别,它们对于酸腐病菌广泛的pH值适应性及促进柑橘果实侵染进程具有重要作用。Studies have shown that Geotrichum citri-aurantii can adjust the pH of infection sites to enhance its pathogenicity,and PacC,a key transcription factor in the pH signal transduction pathway,plays an important role in fungal response to environmental pH changes,but the role of PacC in G.citri-aurantii remains unclear.In this study,two candidate PacC genes were annotated from the genome of G.citri-aurantii and analyzed by bioinformatics methods and their expression patterns under different pH conditions were also studied.The results showed that PacC1 and PacC2 belonged to the C2H2 protein family,and were closely related to the PacC of Penicillium digitatum.PacC1 and PacC2 had differences in chromosomal location,protein molecular mass and isoelectric point.Under in vitro conditions,G.citri-aurantii could adapt to a wide range of pH,and the optimal pH value was found to be 3.0.The expression of PacC1 and PacC2 was regulated by pH,and their expression trends were similar.Under in vivo conditions,the pH of sour rot lesions in‘Miyagawa-wase’mandarin fruit tissue at pH>3.0 decreased significantly with the progression of the disease,while the pH of sour rot lesions in Eureka lemon fruit tissue at pH<3.0 increased significantly,and the relative expression levels of PacC1 and PacC2 showed an increasing trend.In conclusion,it can be seen that the environmental pH affects the growth of G.citri-aurantii,which in turn regulates the environmental pH.Due to the differences in gene structure and physicochemical properties,the degree and mode of pH response are different between the two PacC genes.They play an important role in the wide pH adaptability of G.citri-aurantii and in accelerating its infection process in citrus fruit.

关 键 词：酸腐病菌 PACC 生物信息学 PH值 表达模式 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]