CD166对多发性骨髓瘤RPMI-8226细胞增殖、凋亡及自噬的影响实验研究  

Effects of CD166 on proliferation,apoptosis and autophagy of multiple myeloma RPMI-8226 cells

作　　者：张治业 余醒醒 张璐 翟志敏[2] ZHANG Zhiye;YU Xingxing;ZHANG Lu;ZHAI Zhimin(Department of Hematology,Fuyang Hospital,Anhui Medical University,Fuyang 236000,China)

机构地区：[1]安徽医科大学附属阜阳医院血液内科,安徽阜阳236000 [2]安徽医科大学第二附属医院血液内科,安徽合肥230601

出　　处：《陕西医学杂志》2023年第12期1675-1679,共5页Shaanxi Medical Journal

基　　金：安徽省自然科学基金资助项目(2208085MH217);安徽医科大学科研基金资助项目(2022XKJ212)。

摘　　要：目的:探究CD166对多发性骨髓瘤(MM)RPMI-8226细胞增殖、凋亡及自噬的影响。方法:选取MM细胞RPMI-8226,设计并合成靶向CD166的短发夹RNA(shRNA)和阴性对照(NC)进行细胞转染,分为sh-CD166-1组、sh-CD166-2组和NC组。采用MTT法和平板克隆实验观察CD166对RPMI-8226细胞增殖能力和平板克隆形成能力的影响。利用流式细胞仪检测CD166对RPMI-8226细胞凋亡的影响。采用自噬实验观察CD166对RPMI-8226细胞自噬的影响。此外,将BALB/c裸鼠随机分为NC组和sh-CD166组,通过皮下移植瘤模型评估CD166对RPMI-8226细胞体内成瘤能力的影响。结果:sh-CD166转染后,显著抑制RPMI-8226细胞的增殖能力和平板克隆形成能力(均P<0.05)。与NC组比较,sh-CD166-1组和sh-CD166-2组细胞凋亡率升高(均P<0.05),但sh-CD166-1组和sh-CD166-2组比较差异无统计学意义(均P>0.05)。与NC组比较,sh-CD166组细胞中自噬小体表达量明显减少(P<0.05)。此外,体内移植瘤模型结果表明,sh-CD166组体内成瘤能力比NC组显著降低(P<0.05)。结论:CD166能促进MM细胞RPMI-8226增殖,抑制细胞凋亡,并促进皮下成瘤。Objective:To investigate the effects of CD166 on the proliferation,apoptosis and autophagy of multiple myeloma(MM)RPMI-8226 cells.Methods:MM RPMI-8226 cells were selected,and CD166-targeting short hairpin RNA(shRNA)and negative control(NC)were designed and synthesize for cell transfection,and cells were divided into sh-CD166-1,Sh-CD166-2 and NC groups.MTT assay and plate cloning assay were used to observe the effects of CD166 on the proliferation ability and plate cloning ability of RPMI-8226 cells.The effect of CD166 on apoptosis of RPMI-8226 cells was detected by flow cytometry.The effect of CD166 on autophagy of RPMI-8226 cells was observed by autophagy experiment.In addition,BALB/c nude mice were randomly divided into NC group and sh-CD166 group,and the effect of CD166 on tumor-forming ability of RPMI-8226 cells was evaluated by subcutaneous tumor transplantation model.Results:After transfection with sh-CD166,the proliferation ability and plate cloning ability of RPMI-8226 cells were significantly inhibited(all P<0.05).Compared with NC group,the apoptosis rate of sh-CD166-1 and sh-CD166-1 groups was increased(all P<0.05),but there was no statistical significance between Sh-CD166-1 and SH-CD166-1 groups(all P>0.05).Compared with NC group,the expression of autophagosome in sh-CD166 group was significantly decreased(P<0.05).In addition,the results of in vivo transplantation tumor model showed that the tumor formation ability of sh-CD166 group was significantly lower than that of NC group(P<0.05).Conclusion:CD166 can promote the proliferation of MM RPMI-8226 cells,inhibit cell apoptosis,and promote subcutaneous tumor formation.

关 键 词：多发性骨髓瘤 CD166 增殖 凋亡 自噬 裸鼠 

分 类 号：R733.3[医药卫生—肿瘤]