HPV-DNA分型检测联合液基薄层细胞学检查在宫颈癌筛查中的价值研究  

Study on the value of HPV-DNA typing combined with liquid-based thin layer cytology in cervical cancer screening

作　　者：石洁玉 Shi Jieyu(Department of Obstetrics and Gynecology Jiangyin Ligang Hospital,Jiangyin 214400,China)

机构地区：[1]江阴市利港医院妇产科,江苏江阴214400

出　　处：《实用妇科内分泌电子杂志》2023年第23期88-90,共3页Electronic Journal of Practical Gynecological Endocrinology

摘　　要：目的观察人乳头瘤病毒-DNA(HPV-DNA)分型检测联合液基薄层细胞学检查(TCT)在宫颈癌筛查中的价值。方法选取3000例接受宫颈癌筛查的受检女性作为研究对象,均采取HPV-DNA分型检测、TCT检查,对HPV-DNA分型高危型(+)、TCT(+)或两种检测双(+)者行阴道镜活检组织病理学检查,比较各项检测结果。结果3000例受检者TCT检测、HPV-DNA检测阳性率分别为4.50%(135/3000)、21.67%(650/3000),TCT检测炎性或正常、意义不明的非典型鳞状上皮细胞(ASC-US)、低度鳞状上皮内病变(LSIL)、鳞状上皮高度病变(HSIL)及鳞状细胞癌(SCC)患者HPV-DNA阳性检出率,呈逐渐升高趋势;以阴道镜活检组织病理学检查结果为金标准,宫颈上皮内瘤变(CIN)1级、CIN2级、CIN3级组中,两种检测方法协同检测阳性检出率均显著高于单一方法,差异均有统计学意义(P<0.05);CIN3级、SCC组的两种检测方法协同检测阳性检出率均高于CIN1级组与CIN2级组,但差异均无统计学意义(P>0.05)。结论HPV-DNA分型检测、TCT检测、阴道镜活检组织病理学检查各有优缺点,合理的联合筛查可相互弥补不足。Objective To observe the value of human papillomavirus DNA(HPV-DNA)typing combined with liquid based thin layer cytology(TcT)in cervical cancer screening.Methods 3000 women undergoing cervical cancer screening were selected as the study objects,and HPV-DNA typing test and TCT examination were all adopted.Colposcopic biopsy histopathology examination was performed on those with HPV-DNA typing high risk type(+),TCT(+)or two kinds of detection double(+),and the test results were compared.Results The positive rates of TCT detection and HPV-DNA detection in 3000 subjects were 4.50%(135/3000)and 21.67%(650/3000),respectively.The positive rate of HPV-DNA in patients with inflammatory or normal atypical squamous epithelial cells(ASC-US),low-grade squamous intraepithelial lesions(LSIL),high-grade squamous epithelial lesions(HSIL)and squamous cell carcinoma(SCC)was gradually increased by TCT.Taking colposcopic biopsy histopathology as the gold standard,the positive detection rate of the two methods in the cervical intraepithelial neoplasia(CIN)grade 1,CIN2 and CIN3 groups was significantly higher than that of the single method,with statistical significance(P<0.05).The synergistic positive detection rate of CIN3 and SCC groups was higher than that of CIN1 and CIN2 groups,but the difference was not statistically significant(P>0.05).Conclusion HPV-DNA typing test,TCT test and colposcopic biopsy histopathology have their advantages and disadvantages,and reasonable combined screening can make up for each other's shortcomings.

关 键 词：HPV-DNA分型检测 液基薄层细胞学检查 阴道镜活检组织病理学检查 宫颈癌筛查 

分 类 号：R737.33[医药卫生—肿瘤]