帕金森病患者唾液中α-syn、DJ-1、HO-1、AchE水平变化及其辅助诊断价值  

Changes of α-syn,DJ-1,HO-1 and AchE levels in saliva of patients with Parkinson's disease and their auxiliary diagnostic value

作　　者：雷蕾 王文哲 LEI Lei;WANG Wenzhe(Department of Neurology,Xianyang Hospital,Yan'an University,Xianyang,Shaanxi 712000,China)

机构地区：[1]陕西省延安大学咸阳医院神经内科,陕西咸阳712000

出　　处：《检验医学与临床》2023年第23期3506-3509,共4页Laboratory Medicine and Clinic

摘　　要：目的分析帕金森病患者唾液中α-突触核蛋白(α-syn)、DJ-1、血红素加氧酶-1(HO-1)、乙酰胆碱酯酶(AchE)水平变化及其辅助诊断价值。方法选取2021年8月至2022年8月该院收治的帕金森病患者96例作为帕金森病组,另选取同期在该院体检的健康人群80例作为健康对照组。根据蒙特利尔认知量表(MoCA)评分将帕金森病患者分为合并认知障碍组和无认知障碍组,采集所有受检者的唾液标本,采用酶联免疫吸附试验检测HO-1、α-syn、DJ-1水平,酶比色法检测AChE水平,比较各组受检者的唾液指标水平,采用受试者工作特征(ROC)曲线分析α-syn、DJ-1、HO-1、AchE指标单独及联合诊断帕金森病的效能。结果帕金森病组患者唾液α-syn、DJ-1、HO-1水平低于健康对照组,AchE水平高于健康对照组,差异均有统计学意义(P<0.05)。合并认知障碍组患者唾液α-syn、DJ-1、HO-1水平低于无认知障碍组,AchE水平高于无认知障碍组,差异均有统计学意义(P<0.05)。ROC曲线分析结果显示,α-syn、DJ-1、HO-1、AchE单独检测诊断帕金森病的曲线下面积(AUC)分别为0.723、0.781、0.816、0.671,4项指标联合检测诊断帕金森病的AUC为0.876。结论帕金森病患者唾液中α-syn、DJ-1、HO-1、AchE水平呈明显异常,且与患者认知功能改变相关,4项指标在辅助诊断帕金森病方面具有一定价值。Objective To analyze the changes ofα-synuclein(α-syn),DJ-1,heme oxygenase-1(HO-1)and acetylcholinesterase(AchE)levels in saliva of patients with Parkinson's disease and their auxiliary diagnostic value.Methods A total of 96 patients with Parkinson's disease admitted to this hospital from August 2021 to August 2022 were selected as the Parkinson's disease group,and 80 healthy people who underwent physical examination in this hospital were selected as the healthy control group during the same period.According to Montreal Cognitive Scale(MoCA)score,patients with Parkinson's disease were divided into cognitive impairment group and non-cognitive impairment group.Saliva samples were collected from all subjects,and the levels of HO-1,α-syn and DJ-1 were detected by enzyme-linked immunosorbent assay.AchE level was detected by enzyme colorimetry.The salivary index levels of the subjects were compared,and the receiver operating characteristic(ROC)curve was used to analyze the efficacy ofα-syn,DJ-1,HO-1 and AchE indexes in the diagnosis of Parkinson's disease.Results The levels ofα-syn,DJ-1 and HO-1 in saliva of Parkinson's disease group were lower than those of healthy control group,and the level of AchE in saliva of Parkinson's disease group was higher than that of healthy control group,and the differences were statistically significant(P<0.05).The levels ofα-syn,DJ-1 and HO-1 in saliva of cognitive impairment group were lower than those of non-cognitive impairment group,and the level of AchE of cognitive impairment group was higher than that of non-cognitive impairment group,and the differences were statistically significant(P<0.05).ROC curve analysis showed that the area under the curve(AUC)ofα-syn,DJ-1,HO-1 and AchE in the diagnosis of Parkinson's disease was 0.723,0.781,0.816 and 0.671,respectively.The AUC of combined detection of the 4 indicators in the diagnosis of Parkinson's disease was 0.876.Conclusion The levels of α-syn,DJ-1,HO-1 and AchE in saliva of patients with Parkinson's disease are significantly

关 键 词：帕金森病 唾液 Α-突触核蛋白 DJ-1 血红素加氧酶-1 乙酰胆碱酯酶 

分 类 号：R742.5[医药卫生—神经病学与精神病学]