小球藻的基因工程改造研究进展  

Advances of genetic engineering modification in Chlorella

作　　者：张甜甜 陈必链[1,2] 郑明敏 ZHANG Tiantian;CHEN Bilian;ZHENG Mingmin(College of Life Sciences,Fujian Normal University,Fuzhou 350117,China;Engineering Research Center of Industrial Microbiology,Ministry of Education,Fujian Normal University,Fuzhou 350117,China)

机构地区：[1]福建师范大学生命科学学院,福建福州350117 [2]福建师范大学,工业微生物教育部工程研究中心,福建福州350117

出　　处：《渔业研究》2023年第6期593-602,共10页Journal of Fisheries Research

基　　金：国家自然科学基金项目(31900092);福建省自然科学基金项目(2021J05037)。

摘　　要：小球藻是一种可以利用阳光和二氧化碳进行光合生长并积累生物质的单细胞微藻,被广泛用于生产生物燃料和其他高附加值产品,在水产养殖、食品、能源和环境等领域均有重要应用。随着基因工程技术的发展,小球藻分子水平方面的研究和应用逐渐受到关注,对其进行特定的遗传改造也成为了可能。本文通过从转化系统、转化方法、转化影响因素、外源蛋白表达等四个方面进行总结归纳,阐述了小球藻在基因工程改造领域中的应用和仍存在的难题,期望为小球藻的高附加值产品生产提供理论支持和参考。Chlorella is a unicellular microalga that could grow and accumulate biomass through photosynthesis using sunlight and carbon dioxide.It is widely used to produce biofuels and other high-value products in fields like aquaculture,food,energy,and the environment.With the development of genetic engineering technology,research and applications of Chlorella at the molecular level have received increasing attention,making specific genetic modification possible.In this paper,we summarized and discussed the application of Chlorella in genetic engineering and protein production from four perspectives:transformation system,transformation methods,factors affecting transformation and expression of exogenous proteins.The aim of this review was to provide theoretical support and references for high-value products production of Chlorella.

关 键 词：小球藻 基因工程 转化 蛋白表达 

分 类 号：Q939.9[生物学—微生物学]