红树莓CPP转录因子家族的生物信息学及表达分析  

Bioinformatics and expression analysis of CPP transcription factor family of red raspberry

作　　者：郑奕宸 李明[1] 李闯 吴菁菁 李寒[2] 顾玉红[1] ZHENG Yichen;LI Ming;LI Chuang;WU Jingjing;LI Han;GU Yuhong(College of Life Science,Hebei Agricultural University,Baoding 071001,China;College of Foresty,Hebei Agricultural University,Baoding 071001,China)

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北农业大学林学院,河北保定071001

出　　处：《河北农业大学学报》2023年第6期58-65,共8页Journal of Hebei Agricultural University

基　　金：河北省重点研发计划项目(20326338D)。

摘　　要：为探索CPP转录因子家族在红树莓果实中的功能,本研究通过生物信息学技术与表达分析结合的方法,在‘海尔特兹’红树莓转录组数据库中检索到的CPP转录因子家族进行生物信息学分析、转录表达量分析和qRT-PCR表达分析。结果表明:红树莓CPP基因家族包括4个成员,相对分子质量在47175.57~86763.46 kD之间,等电点在5.83~9.20之间,为不稳定、亲水性的蛋白质,无信号肽,定位于细胞核,无跨膜结构,含有2个CXC保守结构域,结构主要由无规则卷曲构成,含有8~12个外显子,7~8个保守基序,与拟南芥的CPP基因亲缘关系较近,含有生长素、脱落酸、赤霉素、水杨酸、防御和应激、干旱诱导等顺式作用元件,转录表达和qRT-PCR表达均分析发现RuCPP-1和RuCPP-4基因在青果时期的表达量最高,RuCPP-2和RuCPP-3基因分别在红果和深红果时期的表达量最高,推测RuCPP基因家族可能参与调控生长素、脱落酸、赤霉素、水杨酸等反应。In order to explore the function of CPP transcription factor family in red raspberry fruits,this study conducted bioinformatics analysis,transcriptional expression analysis and qRT-PCR expression analysis of CPP transcription factor family retrieved from the‘Heritage’red raspberry transcriptome database.The results showed that the red raspberry CPP gene family consisted of 4 members,whose relative molecular weight ranged from 47175.57 to 86763.46 kD and isoelectric point ranged from 5.83 to 9.20.They were unstable,hydrophilic proteins without signal peptides,and localized on the nucleus without transmembrane structure.The CPP proteins contained 2 CXC conserved domains and the structure were mainly composed of random crimp.The CPP genes contained 8~12 exons and 7~8 conserved motifs.They were closely related to the CPP gene of Arabidopsis thaliana.Cis-acting elements were identified including auxin,abscisic acid,gibberellin,salicylic acid,drought induction,defense and stress responsive elements.Expression analysis showed that the expression levels of RuCPP-1 and RuCPP-4 were the higher in the green fruit stage,and the expression levels of RuCPP-2 and RuCPP-3 were the higher in the red and deep red fruit stages.It was speculated that RuCPP gene family might be involved in the response of auxin,abscisic acid,gibberellin and salicylic acid.

关 键 词：红树莓 CPP转录因子家族 生物信息学分析 表达分析 

分 类 号：Q781[生物学—分子生物学] S663.2[农业科学—果树学]