miR-3680-3p在肾透明细胞癌组织中的表达及与术后复发转移的关系  

Expression of miR-3680-3p in kidney renal clear cell carcinoma and its relationship with postoperative recurrence and metastasis

作　　者：苟东 王全 刘蓉 何星成 龙家才 GOU Dong;WANG Quan;LIU Rong;HE Xingcheng;LONG Jiacai(Department of Urology,Bazhong Central Hospital,Bazhong Sichuan 636000,China)

机构地区：[1]巴中市中心医院泌尿外科,四川巴中636000

出　　处：《新疆医科大学学报》2023年第11期1496-1501,共6页Journal of Xinjiang Medical University

基　　金：四川省卫计委科研计划项目(1931392)。

摘　　要：目的分析微小核糖核酸-3680-3p(miR-3680-3p)在肾透明细胞癌(KIRC)组织中的表达及与术后复发转移的关系。方法选取2015年8月至2018年8月巴中市中心医院收治的肾透明细胞癌患者212例,均采取手术治疗。术中留取肾透明细胞癌病理组织及癌旁组织,利用基因芯片技术筛选miR-3680-3p是否为KIRC组织中差异表达的miRNA分子,使用实时荧光定量PCR(qRT-PCR)进行验证。以肾透明细胞癌病理组织中miR-3680-3p表达水平的中位数为分界,将患者分为低表达组和高表达组。随访至2022年8月,统计KIRC患者复发转移发生情况,分析miR-3680-3p表达与病理学参数的关系。以Kaplan-Meier(K-M)与Cox比例风险回归法分析miR-3680-3p表达状态与预后生存的关系。结果癌组织中的miR-3680-3p相对表达量低于癌旁组织(P<0.05)。随访至2022年8月,有26例患者出现复发转移(12.26%)。miR-3680-3p高表达组患者的肿瘤直径(>10 cm)、肿瘤坏死、TNM分期(Ⅲ~Ⅳ期)、Fuhrman分级(3~4)占比、有淋巴转移占比低于低表达组。K-M分析显示,miR-3680-3p高表达组和低表达组患者的无进展生存期和总生存期差异有统计学意义(P<0.05)。Cox比例风险回归分析显示,TNM分期(Ⅲ~Ⅳ期)、Fuhrman分级(3~4)及miR-3680-3p低表达是影响患者术后复发转移的独立风险因素(P<0.05)。结论miR-3680-3p在KIRC中异常低表达,且对KIRC术后复发转移有较高的预测价值,可作为预测预后可靠生物标志物。Objective To analyze the expression of micro RNA-3680-3p(miR-3680-3p)in kidney renal clear cell carcinoma(KIRC)and its relationship with postoperative recurrence and metastasis.Methods A total of 212 patients with KIRC admitted to the hospital from August 2015 to August 2018 were selected for surgical treatment.The pathological tissues and adjacent tissues of renal clear cell carcinoma were collected during theoperation,and the expression level of miR-3680-3p in the pathological tissue was detected by real-time quantitative PCR(qRT-CR).Thepatients were divided into low expression group and high expression group based on the median expression level of miR-3680-3p in the pathological tissue of renal clear cell carcinoma.The patients were followed up until August 2022,and the incidence of recurrence and metastasis in KIRC patients was analyzed.The relationship between miR-3680-3p expression and pathological parameters was analyzed.Kaplan-Meier(K-M)and Cox proportional hazard regression were used to analyze the relationship between miR-3680-3p expression and survival.Results The relative expression of miR-3680-3p in cancer tissues was lower than that in adjacent tissues(P<0.05).During follow-up until August 2022,26 patients(12.26%)developed recurrence and metastasis.The proportion of tumor diameter(>10 cm),tumor necrosis,TNM stage(Ⅲ~Ⅳstage),Fuhrman grade(3-4)and lymph metastases in the high miR-3680-3p expression group were lower than those in the low expression group(P<0.05).K-M analysis showed that the progression-free survival and overall survival of thepatients with high expression of miR-3680-3p and low expression were significantly different(P<0.05).Cox proportional risk regression analysis showed that TNM stage(Ⅲ~Ⅳstage),Fuhrman grade(3-4)and low expression of miR-3680-3p were independent risk factors for postoperative recurrence and metastasis(P<0.05).Conclusions miR-3680-3p is abnormally low expressed in KIRC,and it has high predictive value for postoperative recurrence and metastasis of KIRC,which c

关 键 词：微小核糖核酸-3680-3p 肾透明细胞癌 复发 转移 

分 类 号：R737.11[医药卫生—肿瘤]