香紫苏醇发酵液中香紫苏醇的提取与纯化工艺  

Extraction and Purification Process of Sclareol from Sclareol Fermentation Broth

作　　者：孙立权[1] 郜玉欣 滕茂浩 SUN Li-quan;GAO Yu-xin;TENG Mao-hao(School of Life Science,Beijing Institute of technology,Beijing 100081,China)

机构地区：[1]北京理工大学生命学院,北京100081

出　　处：《化学试剂》2023年第12期48-53,共6页Chemical Reagents

基　　金：国家自然科学基金面上项目(22176013)。

摘　　要：香紫苏醇作为合成龙涎香的中间体和配制香料的一种重要成分,受到香料领域的大量关注。选取微波辅助法和丙酮-萃取结晶法提取和纯化香紫苏醇发酵液中的香紫苏醇。通过单因素实验和响应面法优化了香紫苏醇的提取工艺,获得最佳提取工艺条件:料液比为1∶25(g/mL),微波温度为59℃,提取时间为9.5 min,在此条件下,菌体的提取率为6.26%。丙酮-萃取结晶法分离纯化提取物得到的香紫苏醇纯度为94.20%,回收率为63.56%。整个提取和纯化工艺过程步骤少,操作简单,所用溶剂可循环利用,对环境友好,实验结果可为发酵法生产香紫苏醇的工业化提供参考和技术支撑。Sclareol is an important component in the synthesis and preparation of perfume,and has been focused by the experts in the spice industry.Microwave-assisted extraction method and recrystallization method with acetone were used for extracting and purifying Sclareol from the fermentation broth of Sclareol.The optimal parameters in the extraction process by single factor experiments and response surface experiment were obtained as follows:the solid-liquid ratio was 1∶25(g/mL),the microwave temperature was 59℃,and the extraction time was 9.5 min.The extraction yield of crude Sclareol was 6.26%.The crude product was separated and purified by recrystallization method with acetone,and the purity of recrystallized Sclareol was higher than 94.20%,and the recovery yield was near to 63.56%.The whole extraction and purification process is environmentally friendly including a few steps of simple operation,and the solvent used can be recycled.The experimental results show more evidence for industrial production of Sclareol by fermentation method.

关 键 词：香紫苏醇 发酵液 提取 响应面法 纯化 

分 类 号：R284.2[医药卫生—中药学]