反相高效液相色谱法测定乳粉中6S-5-甲基四氢叶酸钙含量  

Determination of 6S-5-Methyltetrahydrofolate Calcium in Milk Powder by Reversed-Phase High Performance Liquid Chromatography

作　　者：刘丽君 陈静 段国霞 李翠枝 吕志勇 LIU Lijun;CHEN Jing;DUAN Guoxia;LI Cuizhi;LÜZhiyong(Key Laboratory of Milk and Dairy Products Detection and Monitoring Technology for State Market Regulation,Inner Mongolia Yili Industrial Group Co.Ltd.,Hohhot 010110,China)

机构地区：[1]内蒙古伊利实业集团股份有限公司,国家市场监管重点实验室(乳及乳制品检测与监控技术),内蒙古呼和浩特010110

出　　处：《乳业科学与技术》2023年第4期35-39,共5页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金：内蒙古自治区重点研发和成果转化计划项目(2023YFHH0093)。

摘　　要：建立乳粉中6S-5-甲基四氢叶酸钙的反相高效液相色谱分析方法。使用三氯乙酸溶液沉淀蛋白,抗坏血酸溶液加热提取后,以C_(18)反相色谱柱分离,三氟乙酸溶液(0.1%)-甲醇为流动相,采用梯度方式洗脱,检测波长280 nm,单标法定量。结果表明:乳粉样品中6S-5-甲基四氢叶酸钙的定量限为150μg/100 g,检出限为50μg/100 g;乳粉中6S-5-甲基四氢叶酸钙的添加量为150~600?μg/100 g时,加标回收率为95.0%~109.1%,相对标准偏差为1.26%~4.04%。本方法操作简便、准确、回收率高、重复性好,适用于乳粉中6S-5-甲基四氢叶酸钙的含量测定。A reversed-phase high performance liquid chromatography(RP-HPLC)method was established for the determination of 6S-5-methyltetrahydrofolate calcium in milk powder.After protein precipitation with trichloroacetic acid solution,samples were extracted with ascorbic acid solution in a hot water bath,and the extract was separated on a C18 reversed-phase column by gradient elution using a mobile phase composed of 0.1%trifluoroacetic acid solution and methanol,and the detection wavelength was set at 280 nm.Quantitative analysis was carried out by the single standard method.The results showed that the limit of quantification and detection of 6S-5-methyltetrahydrofolate calcium in milk powder samples were 150 and 50μg/100 g,respectively.The recoveries for samples spiked at levels ranging from 150 to 600μg/100 g were 95.0%–109.1%with relative standard deviations(RSDs)between 1.26%and 4.04%.This method is simple,accurate,with high recovery and reproducibility,and suitable for the determination of 6S-5-methyltetrahydrofolate calcium in milk powder.

关 键 词：乳粉 反相高效液相色谱法 6S-5-甲基四氢叶酸钙 三氯乙酸 抗坏血酸 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]