基于公共数据库分析VSIG4在结直肠癌中的表达及意义  

Public database analysis of VSIG4 expression and immune mechanism in colorec⁃tal cancer

作　　者：李辉[1] 马辉[1] 温佳颖 唐宇星 黄志广 Li Hui;Ma Hui;Wen Jiaying;Tang Yuxing;Huang Zhiguang(Department of Colorectal and Anal Surgery,First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China;Department of Radiotherapy,Second Affiliated Hospital of Guangxi Medical University,Nanning 530007,Guangxi Zhuang Auton⁃omous Region,China;Department of Pathology,First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autono⁃mous Region,China)

机构地区：[1]广西医科大学第一附属医院结直肠肛门外科,广西南宁530021 [2]广西医科大学第二附属医院放疗科,广西南宁530007 [3]广西医科大学第一附属医院病理科,广西南宁530021

出　　处：《结直肠肛门外科》2023年第5期491-501,共11页Journal of Colorectal & Anal Surgery

基　　金：广西壮族自治区卫生健康委员会自筹经费科研课题(Z-A20220415);广西医科大学青年科学基金项目(GXMUYSF201914)。

摘　　要：目的 研究VSIG4在结直肠癌(CRC)中的表达及其对患者预后的影响,并探索VSIG4表达在CRC中与免疫细胞浸润的关系。方法 本研究对来源于多个公共数据库中的VSIG4 mRNA表达数据进行汇总分析。GO、KEGG和GSEA富集分析用于进一步探索VSIG4在CRC中的生物学作用和参与的信号通路。通过CIBERSORT算法分析VSIG4与免疫细胞浸润的关系,进一步探讨VSIG4的表达对CRC患者预后的影响。结果 基于3 524例样本的汇总分析证实VSIG4在CRC中表达下调(SMD=-0.60)。SROC曲线分析提示VSIG4可较好地区分CRC样本和非癌对照组样本(AUC=0.75)。GO、KEGG和GSEA富集分析提示VSIG4主要通过调节信号受体的结合与活性从而调节肿瘤免疫反应。CIBERSORT分析表明VSIG4高/低表达两组CRC样本间免疫细胞浸润有显著性差异。基于970例CRC患者总生存期数据的汇总分析提示VSIG4的表达情况与CRC患者预后显著相关。结论 本研究证实VSIG4在CRC中表达下调,并通过调节免疫反应参与CRC的发展过程,从而影响患者预后。Objectives To investigate the expression and patient prognosis of VSIG4 in colorectal cancer(CRC)and explore the relationship between VSIG4 expression and immune cell infiltration in CRC.Methods This study compiled and analyzed VSIG4 mRNA expression data from various public databases.Additionally,GO,KEGG,and GSEA enrichment analysis were used to fur-ther explore the biological role and signaling pathways of VSIG4 in CRC.Subsequently,the CIBERSORT algorithm was used to analyzed relationship between VSIG4 and immune cell infiltration,and the impact of VSIG4 expression on the prognosis of CRC patients was further discussed.Results Utilizing a total of 3524 samples,the meta-analysis substantiated the down-regulation of VSIG4 in CRC with a standard mean difference(SMD)of-0.60.Receiver operating characteristic(SROC)curve analysis suggest-ed that VSIG4 effectively distinguishs CRC samples from non-cancer control samples(AUC=0.75).GO,KEGG,and GSEA en-richment analysis proposed that VSIG4 primarily regulates tumor immune responses by modulating the binding and activity of sig-naling receptors.CIBERSORT analysis revealed significant differences in immune cell infiltration between VSIG4 high/low expres-sion groups in CRC samples.Drawing on a total of 970 CRC patient survival data,the meta-analysis suggested a significant asso-ciation between VSIG4 expression and CRC patient prognosis.Conclusion This study affirmsthe down-regulation of VSIG4 in CRC and establishes its role in CRC development through the regulation of immune responses,consequently influencing the pa-tient prognosis.

关 键 词：结直肠癌 VSIG4 肿瘤免疫微环境 

分 类 号：R735.3[医药卫生—肿瘤]