酵母中乙酰辅酶A的代谢工程研究进展  

Advance in metabolic engineering of acetyl-CoA in yeast

作　　者：王招悬 刘营航 刘萌萌[1] 祁庆生[1] WANG Zhaoxuan;LIU Yinghang;LIU Mengmeng;QI Qingsheng(State Key Laboratory of Microbial Technology,Shandong University,Qingdao 266200,China)

机构地区：[1]山东大学微生物技术国家重点实验室(微生物技术研究院),山东青岛266200

出　　处：《生物加工过程》2023年第6期599-607,共9页Chinese Journal of Bioprocess Engineering

基　　金：国家重点研发计划(2021YFC2100500);山东省重大创新工程项目(2020CXGC010602)。

摘　　要：乙酰辅酶A是微生物代谢途径中的关键节点,参与胞内多种化合物的生物合成。通过代谢工程改造乙酰辅酶A的生产途径,增加乙酰辅酶A产量,是提高以乙酰辅酶A为前体化合物产量的重要手段。本文介绍了酵母胞内乙酰辅酶A合成途径,详细阐述了丙酮酸脱氢酶(PDH)旁路途径、PDH途径、磷酸转酮酶-磷酸转乙酰酶(PK-PTA)途径和柠檬酸裂解途径生成乙酰辅酶A的过程以及乙酰辅酶A合成途径在酿酒酵母和解脂耶氏酵母中的不同,总结了酵母中乙酰辅酶A合成途径的代谢调控策略以及2种酵母改造策略的相互借鉴,为最终在酵母中实现目标化合物的高效生产提供指导。Acetyl-CoA,a key node in microbial metabolic pathways,is involved in the intracellular biosynthesis of a variety of chemicals.The modification of production pathway of acetyl-CoA through metabolic engineering methods can potentially increase the acetyl-CoA level,thus becomes an important approach to improve the yields of compounds with acetyl-CoA as a synthetic precursor.In this paper,we first presented various pathways of the intracellular acetyl-CoA synthesis,including PDH bypass pathway,PDH pathway,PK-PTA pathway and citrate cleavage pathway,as well as their differences in Saccharomyces cerevisiae and Yarrowia lipolytica.Then we summarized several strategies for the metabolic regulation of acetyl CoA synthesis pathway in yeast,along with their comparison in two kinds of yeast.This review will provide useful guidance for the efficient production of target compounds in yeast.

关 键 词：乙酰辅酶A 酵母 代谢工程 

分 类 号：Q939.9[生物学—微生物学]