苹果茎沟病毒柑橘碎叶株系的分布与序列分析  

Distribution and molecular characterization of citrus tatter leaf virus in China

作　　者：高海馨 陈香玲[3] 乔兴华 廖慧红 周常勇 周彦 Gao Haixin;Chen Xiangling;Qiao Xinghua;Liao Huihong;Zhou Changyong;Zhou Yan(Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City,Chongqing 400000,China;National Citrus Engineering Research Center,Citrus Research Institute,Southwest University,Chongqing 400712,China;Horticultural Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi Zhuang Autonomous Region,China;Plant Protection and Fruit Tree Technology Popularization Station in Wanzhou District of Chongqing City,Wanzhou 404199,Chongqing,China)

机构地区：[1]西部(重庆)科学城种质创制大科学中心,重庆400000 [2]西南大学柑桔研究所国家柑桔工程技术研究中心,重庆400712 [3]广西农业科学院园艺研究所,南宁530007 [4]重庆市万州区植物保护与果树技术推广站,万州404199

出　　处：《植物保护学报》2023年第5期1318-1326,共9页Journal of Plant Protection

基　　金：财政部和农业农村部国家现代农业产业技术体系(CARS-26-05B)。

摘　　要：为明确苹果茎沟病毒(apple stem grooving virus,ASGV)柑橘碎叶株系(citrus tatter leaf virus,CTLV)在中国柑橘产区的发生分布及其分子特性,于2017—2021年对我国12个柑橘主产区开展系统调查,并采用多重比对以及系统发育分析法对CTLV的全序列进行分析。结果表明,从12个柑橘主产区采集的2012份疑似样品中检测出413份感CTLV阳性样品。除陕西省外,其余11个柑橘主产区样品中均检测出CTLV,并且柚类样品中CTLV的检出率最高,达到32.31%。对分离获得的22株CTLV毒株和已知的7株CTLV以及5株ASGV毒株进行全序列分析,发现所有34株毒株的核苷酸序列相似性为78.6%~99.8%,且CTLV序列的保守性较高。此外,与其他4株ASGV毒株相比,本研究中22株CTLV毒株与ASGV-MK毒株(GenBank登录号为MZ127820.1)具有更近的亲缘关系。推测CTLV中国毒株间的亲缘关系可能与其采样地和寄主品种有关。To better understand the distribution and molecular characteristics of citrus tatter leaf virus(CTLV)in China,a field survey was conducted from 2017 to 2021,and a total of 2012 citrus samples were collected from 12 Chinese major citrus-growing provinces.Among these,413 citrus samples were tested positive for CTLV,and this virus was present in all of the Chinese major citrus-growing provinces,except Shaanxi Province.In surveyed citrus genotypes,the most frequently infected genotype was pummelo,with an infection rate of 32.31%.CTLV is a strain of apple stem grooving virus(ASGV,Capillovirus).In this study,the complete genome sequences of 22 CTLV isolates from different provinces and hosts were sequenced.Comparisons of the whole-genome sequences of these 22 CTLV isolates as well as seven CTLV and five ASGV isolates previously reported from around the world revealed that the sequence identity ranged from 78.6%to 99.8%,indicating that there was a very low level of sequence heterogeneity among CTLV isolates of different geographic origins and hosts.Phylogenetic analyses of these 34 genomic sequences suggest that ASGV-MK(GenBank accession no.:MZ127820.1)has a closer relationship with CTLV than other four ASGV isolates.These results indicate that the evolutionary relationships of Chinese CTLV isolates may be associated with geographic origin and host species.

关 键 词：苹果茎沟病毒 柑橘碎叶株系 检测 序列分析 系统进化分析 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]