硒锌对老化过程中Aβ所致线粒体损伤的保护作用及机制  

PROTECTION AND MECHANISM OF SELENIUM AND ZINC IN AβINDUCED MITOCHONDRIAL DAMAGE DURING AGING

作　　者：田原瑗 赵晨晨 姜勃宇 许妍姬[1] TIAN Yuan-yuan;ZHAO Chen-chen;JIANG Bo-yu;XU Yan-ji(Department of Preventive Medicine,Medical College Yan bian University,Yanji 133000,China)

机构地区：[1]延边大学医学院预防医学教研室,延吉133000

出　　处：《营养学报》2023年第5期453-459,共7页Acta Nutrimenta Sinica

基　　金：吉林省教育厅“十三五”科学技术项目(No.JJKH20200532KJ)。

摘　　要：目的探讨硒(Zn)锌(Se)联合使用对老化过程中Aβ所致线粒体损伤的保护作用及其可能机制。方法在体外实验中,使用10μmol/L Aβ25-35诱导HT22细胞构建衰老模型,分为对照(CT)、模型(AE)、AE+Zn(0.1μmol/L Zn)、AE+Se(0.1μmol/L Se)、AE+Zn+Se(0.1μmol/L Zn、0.1μmol/L Se)组。24 h内,用相关试剂盒检测Na+K+-ATP酶、呼吸链复合物I、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性,丙二醛(MDA)、谷胱甘肽(GSH)含量,线粒体中Aβ1-42含量;Western blot(WB)法检测Cofilin蛋白表达水平后进行Image J灰度分析。在体内试验中,将60只2月龄雄性昆明小鼠随机分为CT、AE、AE+Zn、AE+Se、AE+Zn+Se及阳性对照6个组。连续53 d腹腔注射400 mg/kg D-半乳糖建立衰老模型,应用水迷宫和被动回避反应实验检测Zn、Se对衰老模型小鼠的学习记忆能力的影响。24 h内,检测Na+K+-ATP酶、呼吸链复合物I、CAT、SOD,MDA、GSH、脑组织线粒体中Aβ1-42含量;WB检测脑组织中Cofilin蛋白表达水平后进行Image J灰度分析。结果体内、外结果显示,Zn、Se、Zn+Se组Na+K+-ATP酶、呼吸链复合物I、CAT、SOD活力均升高,MDA含量降低,GSH含量上升,Cofilin的蛋白表达水平下调,Aβ1-42含量降低,Zn+Se效果优于单独使用组(P<0.05)。水迷宫实验显示,Zn、Se、Zn+Se组逃避潜伏期显著缩短,在目标象限中总时间占比和穿越平台次数明显增加;被动回避反应实验显示,Zn、Se、Zn+Se组潜伏期延长,错误次数减少(P<0.05)。结论Zn、Se对老化过程中Aβ所致线粒体损伤有保护作用,并使衰老模型小鼠学习和空间记忆能力得到改善。Objective To explore protective effect and possible mechanism of zinc(Zn)and selenium(Se)in Aβinduced mitochondriae damage in aging process.Method In vitro experiments were carried out in 10μmol/L Aβ25-35 exposed HT22 cells.The cells were assigned to five groups:control(CT),age(AE),AE+Zn(0.1μmol/L Zn),AE+Se(0.1μmol/L Se)and AE+Zn+Se(0.1μmol/L Zn and 0.1μmol/L Se).The activities of Na+K+-ATPase,respiratory chain complex I,catalase(CAT)and superoxide dismutase(SOD)were tested.Malondialdehyde(MDA),glutathione(GSH)and Aβ1-42 in mitochondria were measured.Cofilin protein expression level were characterized by Western blot(WB).In vivo experiments were performed in 60 two-month-old male Kunming mice.They were randomly divided into 6 groups:CT,AE,AE+Zn,AE+Se,AE+Zn+Se and positive control.They were injected intraperitoneally with 400 mg/kg D-galactose for 53 days to develop aging and proccess.The effects of Zn and Se on learning and memory were tested by water maze and passive avoidance Na+K+-ATPase enzyme,respiratory chain complex I,CAT,SOD,MDA,GSH and Aβ1-42 were measured in brain mitochondriae.Cofilin protein expression level in brain tissue was characterized by WB.Results Similar changes were found in both in vivo and in vitro experiments that,an increase in activities of Na+K+-ATPase(in all intervening groups including Zn,Se and Zn+Se),complex I,CAT and SOD,a decrease of MDA concentration,an increase of GSH concentration,a decrease of Cofilin protein expression and Aβ1-42 concentration.The effects of Zn+Se group were better than other groups(P<0.05).The water maze test that the escape latency was significantly shortened in the test groups(Zn,Se and Zn+Se),in which the proportion of total time in the target quadrant and the frequency of crossing the platform were significantly increased.The latency in passive avoidance response experiment was prolonged in the test groups(Zn,Se and Zn+Se)and the number of errors reduced(P<0.05).Conclusion Zn and Se have protective effects on mitochondrial damage caused by Aβ

关 键 词：锌 硒 线粒体 丝切蛋白 

分 类 号：R151.2[医药卫生—营养与食品卫生学]