lncRNA SNHG15通过调控miR-942-5p表达减轻Aβ25-35诱导的PC12细胞氧化应激和细胞凋亡的研究  

lncRNA SNHG15 attenuates Aβ25-35-induced oxidative stress and apoptosis in PC12 cells by regulating the expression of miR-942-5p

作　　者：张文娟 戴婷丽 李沛[3] ZHANG Wenjuan;DAI Tingli;LI Pei(Department of Health Management,Qinghai Provincial People's Hospital,Xining,Qinghai 810000,China;Department of Pathology,Qinghai Provincial People's Hospital,Xining,Qinghai 810000,China;Department of Neurology,Qinghai Provincial People's Hospital,Xining,Qinghai 810000,China)

机构地区：[1]青海省人民医院健康管理部,青海西宁810000 [2]青海省人民医院病理科,青海西宁810000 [3]青海省人民医院神经内科,青海西宁810000

出　　处：《国际检验医学杂志》2023年第23期2880-2885,共6页International Journal of Laboratory Medicine

基　　金：青海省卫生健康委员会指导性计划课题(2019-WJZDX-01)。

摘　　要：目的探讨长链非编码RNA(lncRNA)小核仁RNA宿主基因15(SNHG15)对β淀粉样蛋白_(25-35)(Aβ_(25-35))诱导的PC12细胞氧化应激和细胞凋亡的影响及分子机制。方法将PC12细胞分为Con组、Aβ_(25-35)组、Aβ_(25-35)+si-SNHG15组、Aβ_(25-35)+si-NC组、Aβ_(25-35)+微小RNA(miR)-942-5p组、Aβ_(25-35)+miR-NC组、Aβ_(25-35)+si-SNHG15+anti-miR-942-5p组、Aβ_(25-35)+si-SNHG15+anti-miR-NC组;实时荧光定量PCR(qRT-PCR)检测lncRNA SNHG15和miR-942-5p表达水平;酶联免疫吸附试验检测细胞超氧化物歧化酶(SOD)活性和丙二醛(MDA)表达水平;蛋白质印迹法检测蛋白表达;流式细胞术检测细胞凋亡;双荧光素酶报告实验检测lncRNA SNHG15和miR-942-5p靶向关系。结果Aβ_(25-35)诱导的PC12细胞中lncRNA SNHG15、MDA、Bax表达水平及细胞凋亡率升高,SOD活性、miR-942-5p、Bcl-2表达水平降低(P<0.05)。抑制lncRNA SNHG15表达或过表达miR-942-5p后,SOD活性、Bcl-2表达水平升高,细胞凋亡率、MDA、Bax表达水平降低(P<0.05)。lncRNA SNHG15靶向调控miR-942-5p。下调miR-942-5p表达逆转了抑制lncRNA SNHG15表达对Aβ_(25-35)诱导的PC12细胞氧化应激和凋亡的作用。结论抑制lncRNA SNHG15表达通过上调miR-942-5p表达减轻Aβ_(25-35)诱导的PC12细胞氧化应激和细胞凋亡。Objective To explore the effect of long non-coding RNA small nucleolar RNA host gene 15(lncRNA SNHG15)on the oxidative stress and apoptosis of PC12 cells induced byβ-amyloid protein _(25-35)(Aβ_(25-35))and its molecular mechanism.Methods PC12 cells were divided into Con group,Aβ_(25-35) group,Aβ_(25-35)+si-SNHG15 group,Aβ_(25-35)+si-NC group,Aβ_(25-35)+microRNA(miR)-942-5p group,Aβ_(25-35)+miR-NC group,Aβ_(25-35)+si-SNHG15+anti-miR-942-5p group,Aβ_(25-35)+si-SNHG15+anti-miR-NC group.The expression levels of lncRNA SNHG15 and miR-942-5p were detected by real-time fluorescent quantitative PCR(qRTPCR).Cell superoxide dismutase(SOD)activity and malondialdehyde(MDA)expression level were detected by enzyme-linked immunosorbent assay.Western blot was used to detect protein expression.Cell apoptosis was detected by flow cytometry.The targets relationship of lncRNA SNHG15 and miR-942-5p was detected by dual luciferase reporter assay.Results In PC12 cells induced by Aβ_(25-35),the expression levels of lncRNA SNHG15,MDA,Bax and the apoptosis rate were increased,while the SOD activity,miR-942-5p and Bcl-2 expression levels were decreased(P<0.05).After inhibiting the expression of lncRNA SNHG15 or overexpressing miR-942-5p,SOD activity and Bcl-2 expression level were increased,while apoptosis rate,MDA and Bax expression levels were decreased(P<0.05).lncRNA SNHG15 targeted miR-942-5p.Down-regulating the expression of miR-942-5p reversed the effect of inhibiting the expression of lncRNA SNHG15 on the oxidative stress and apoptosis of PC12 cells induced by Aβ_(25-35).Conclusion Inhibition of lncRNA SNHG15 expression could reduce oxidative stress and apoptosis induced by Aβ_(25-35) in PC12 cells by up-regulating the expression of miR-942-5p.

关 键 词：长链非编码RNA小核仁RNA宿主基因15 微小RNA-942-5p 氧化应激 细胞凋亡 

分 类 号：R741.02[医药卫生—神经病学与精神病学]