Pien Tze Huang alleviates Concanavalin A-induced autoimmune hepatitis by regulating intestinal microbiota and memory regulatory T cells  

作　　者：Xin Zeng Miao-Hua Liu Yi Xiong Lin-Xin Zheng Kai-En Guo Hai-Mei Zhao Yu-Ting Yin Duan-Yong Liu Bu-Gao Zhou 

机构地区：[1]Department of Postgraduate,Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi Province,China [2]College of Traditional Chinese Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi Province,China [3]College of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi Province,China [4]Formula-Pattern Research Center,Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi Province,China [5]Office of Academic Research,Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi Province,China

出　　处：《World Journal of Gastroenterology》2023年第45期5988-6016,共29页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China,No.81603402,82060798,81860791;the Special Fund Project for Graduate Innovation of Jiangxi University of Chinese Medicine,No.JZYC22S77;a Special Fund Project for Graduate Innovation of Jiangxi Province,No.YC2022-s840,YC2022-B188;Jiangxi University of Chinese Medicine Science and Technology Innovation Team Development Program,No.CXTD22008;the Young and Middle-aged Backbone Talent Project of Jiangxi Administration of Traditional Chinese Medicine,No.[2020]05;Young Qhuang Scholars support Project of National Administration of Traditional Chinese Medicine,No.[2022]256.

摘　　要：BACKGROUND Traditional Chinese medicine has used the drug Pien Tze Huang(PTH),a classic prescription,to treat autoimmune hepatitis(AIH).However,the precise mode of action is still unknown.AIM To investigate the mechanism of PTH in an AIH mouse model by determining the changes in gut microbiota structure and memory regulatory T(mTreg)cells functional levels.METHODS Following induction of the AIH mouse model induced by Concanavalin A(Con A),prophylactic administration of PTH was given for 10 d.The levels of mTreg cells were measured by flow cytometry,and intestinal microbiota was analyzed by 16S rRNA analysis,while western blotting was used to identify activation of the toll-like receptor(TLR)2,TLR4/nuclear factor-κB(NF-κB),and CXCL16/CXCR6 signaling pathways.RESULTS In the liver of mice with AIH,PTH relieved the pathological damage and reduced the numbers of T helper type 17 cells and interferon-γ,tumor necrosis factor-alpha,interleukin(IL)-1β,IL-2,IL-6,and IL-21 expression.Simultaneously,PTH stimulated the abundance of helpful bacteria,promoted activation of the TLR2 signal,which may enhance Treg/mTreg cells quantity to produce IL-10,and suppressed activation of the TLR4/NF-κB and CXCL16/CXCR6 signaling pathways.CONCLUSION PTH regulates intestinal microbiota balance and restores mTreg cells to alleviate experimental AIH,which is closely related to the TLR/CXCL16/CXCR6/NF-κB signaling pathway.

关 键 词：Pien Tze Huang Autoimmune hepatitis Intestinal microbiota Memory regulatory T cell Toll-like receptor signaling 

分 类 号：R575[医药卫生—消化系统]