Location-based prediction model for Crohn’s disease regarding a novel serological marker,anti-chitinase 3-like 1 autoantibodies  

作　　者：Nora Sipeki Patricia Julianna Kovats Claudia Deutschmann Peter Schierack Dirk Roggenbuck Maria Papp 

机构地区：[1]Division of Gastroenterology,Department of Internal Medicine,Faculty of Medicine,University of Debrecen,Debrecen H-4032,Hungary [2]Kálmán Laki Doctoral School,Faculty of Medicine,University of Debrecen,Debrecen H-4032,Hungary [3]Institute of Biotechnology,Faculty Environment and Natural Sciences,Brandenburg University of Technology Cottbus-Senftenberg,Senftenberg 01968,Germany [4]Faculty of Health Sciences Brandenburg,Brandenburg University of Technology Cottbus-Senftenberg,Senftenberg 01968,Germany [5]Medipan GmbH&GA Generic Assays GmbH,Dahlewitz-Berlin 15827,Germany

出　　处：《World Journal of Gastroenterology》2023年第42期5728-5750,共23页世界胃肠病学杂志（英文版）

基　　金：Supported by the German Federal Ministry of Education and Research(BMBF-Wachstumskern-PRAEMED.BIO),03WKDB2C;supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences,BO/00232/17/5;Research Grants of National Research Development and Innovation Office,K115818/2015/1;New National Excellence Program of the Ministry of Human Capacities,ÚNKP-18-4 Bolyai Plus.

摘　　要：BACKGROUND Defective neutrophil regulation in inflammatory bowel disease(IBD)is thought to play an important role in the onset or manifestation of IBD,as it could lead to damage of the intestinal mucosal barrier by the infiltration of neutrophils in the inflamed mucosa and the accumulation of pathogens.Like neutrophils in the context of innate immune responses,immunoglobulin A(IgA)as an acquired immune response partakes in the defense of the intestinal epithelium.Under normal conditions,IgA contributes to the elimination of microbes,but in connection with the loss of tolerance to chitinase 3-like 1(CHI3L1)in IBD,IgA could participate in CHI3L1-mediated improved adhesion and invasion of potentially pathogenic microorganisms.The tolerance brake to CHI3L1 and the occurrence of IgA autoantibodies to this particular target,the exact role and underlying mechanisms of CHI3L1 in the pathogenesis of IBD are still unclear.AIM To determine the predictive potential of Ig subtypes of a novel serological marker,anti-CHI3L1 autoantibodies(aCHI3L1)in determining the disease phenotype,therapeutic strategy and long-term disease course in a prospective referral cohort of adult IBD patients.METHODS Sera of 257 Crohn’s disease(CD)and 180 ulcerative colitis(UC)patients from a tertiary IBD referral center of Hungary(Division of Gastroenterology,Department of Internal Medicine,Faculty of Medicine,University of Debrecen)were assayed for IgG,IgA,and secretory IgA(sIgA)type aCHI3L1 by enzyme-linked immunosorbent assay using recombinant CHI3L1,along with 86 healthy controls(HCONT).RESULTS The IgA type was more prevalent in CD than in UC(29.2%vs 11.1%)or HCONT(2.83%;P<0.0001 for both).However,sIgA subtype aCHI3L1 positivity was higher in both CD and UC patients than in HCONT(39.3%and 32.8%vs 4.65%,respectively;P<0.0001).The presence of both IgA and sIgA aCHI3L1 antibodies was associated with colonic involvement(P<0.0001 and P=0.038,respectively)in patients with CD.Complicated disease behavior at sample procurement was associated with aCHI3

关 键 词：Chitinase 3-like 1 autoantibodies Crohn’s disease Ulcerative colitis Disease progression Immunoglobulin subtypes Enzyme-linked immunosorbent assay 

分 类 号：R574.62[医药卫生—消化系统]