基于噬菌体的食源性致病菌检测技术研究进展  

Advances in bacteriophage technology for foodborne pathogenic bacteria detection

作　　者：王璇 戚敏钰 马荟琳 姚一青 陆斌 曹岩[1] WANG Xuan;QI Min-yu;MA Hui-lin;YAO Yi-qing;LU Bin;CAO Yan(Department of Biochemical Pharmacy,Faculty of Pharmacy,Naval Medical University(The Second Military Medical University),Shanghai 200433,China;The Sixth Student Team,College of Basic Medical Sciences,Naval Medical University(The Second Military Medical University),Shanghai 200433,China)

机构地区：[1]海军军医大学(第二军医大学)药学系生化药学教研室,上海200433 [2]海军军医大学(第二军医大学)基础医学院学员六大队,上海200433

出　　处：《药物分析杂志》2023年第10期1645-1652,共8页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(82174092);上海市自然科学基金(21ZR1483000);上海市浦江人才计划(21PJD083)。

摘　　要：食源性致病菌导致的食源性疾病给经济社会发展和人类健康造成了巨大损失,对致病菌的准确检测是预防和治疗这类疾病的必要前提。目前常用的细菌检测技术包括细菌培养法、分子生物学法、免疫学法、仪器法、噬菌体法等。噬菌体具有特异性强,结构简单,价格低廉以及能区分活细菌和死细菌的特性,为开发食源性致病菌的检测技术提供了新思路。本文对基于噬菌体的细菌检测技术的主要特点、基本原理和研究进展进行了综述,为建立快速、有效、经济的细菌检测方法提供参考。Foodborne diseases caused by foodborne pathogenic bacteria have exerted a huge impact not only on the development of economics and society but also on public health.The accurate detection methods for pathogenic bacteria serve as a significant prerequisite in the prevention and treatment of such diseases.At present,some approaches have been applied in the detection of pathogenic bacteria,including bacterial culture assays,molecular biological assays,immunological assays,instrument-based methods,and bacteriophage-based methods.Bacteriophage is highly specific,simple-structured,cost-effective,and available in the recognition of living and dead bacteria,which makes it a promising choice in the field of bacteria detection.In this review,the characteristics,principles,and recent advances of phage-based bacteria detection are presented a comprehensive overview,and opt to provide a basis for further development of rapid,effective,and economical pathogenic bacteria detection methods.

关 键 词：食源性病原菌 细菌 噬菌体 检测方法 生物传感器 荧光标记法 报告基因法 综述 

分 类 号：R917[医药卫生—药物分析学]