比较代谢组学分析桑枝屑栽培对瓦尼桑黄代谢的影响  

Analysis of impact of mulberry sawdust on the metabolism of Sanghuangporus vaninii based on comparative metabolomics

作　　者：宋吉玲[1] 闫静[1] 陆娜[1] 程俊文[2] 周祖法[1] 林佳瑶 王伟科[1] 袁卫东[1] SONG Jiling;YAN Jing;LU Na;CHENG Junwen;ZHOU Zufa;LIN Jiayao;WANG Weike;YUAN Weidong(Hangzhou Acdemy of Agricultural Sciences,Hangzhou 310024,Zhejiang,China;Zhejiang Academy of Forestry,Hangzhou 310023,Zhejiang,China)

机构地区：[1]杭州市农业科学研究院,浙江杭州310024 [2]浙江省林业科学研究院,浙江杭州310023

出　　处：《菌物学报》2023年第11期2231-2243,共13页Mycosystema

基　　金：现代农业产业技术体系建设专项资金资助项目(CARS-20);国家自然科学基金(31501815);浙江省科技厅项目(2021F1065-8,2021C02073-7);杭州市财政项目(2023JXZC-01)。

摘　　要：本研究以杂木屑及桑枝屑栽培的瓦尼桑黄子实体为研究对象,基于液质联用技术检测出代谢产物共2996种,其中差异代谢物628种,上调435种,下调193种,主要集中在有机酸及其衍生物,有机杂环化合物、脂质和类脂分子、有机含氧化合物、苯环型化合物、苯丙素类和聚酮类、核苷酸及其类似物、氨基酸和糖苷类等。KEEG代谢通路分析结果表明,差异显著的代谢通路有5条,分别为磷酸戊糖途径、果糖和甘露糖代谢、糖基磷脂酰肌醇(GPI)锚生物合成、硫胺素代谢和嘧啶代谢,主要围绕糖类物质代谢为主。通过本研究发现,桑枝屑的添加对瓦尼桑黄子实体中次级代谢产物的合成和累积具有显著的促进作用,为瓦尼桑黄的精准化栽培和开发利用提供技术支撑。Based on the analysis of liquid chromatography-mass spectrometry,2996 metabolites were detected from basidiomata of Sanghuangporus vaninii cultivated with mixed sawdust and mulberry sawdust,including 628 differential metabolites of which 435 were upregulated and 193 downregulated.The metabolites were mainly organic acids and their derivatives,organic heterocyclic compounds,lipids and lipoid molecules,organic oxygen-containing compounds,benzene ring compounds,phenylpropanoids and polyketides,nucleotides and their analogs,amino acids,and glycosides.KEEG metabolic pathway analyses showed that there were 5 significantly different metabolic pathways primarily focusing on carbohydrate metabolism,including pentose phosphate pathway,fructose and mannose metabolism,glycosylphosphatidylinositol(GPI)anchor biosynthesis,thiamine metabolism,and pyrimidine metabolism.It was found that the addition of mulberry sawdust could significantly promote the synthesis and accumulation of secondary metabolites in the basidiomata of S.vaninii,and thereby this result provides technical support for the precise cultivation and development of S.vaninii.

关 键 词：瓦尼桑黄 桑枝屑 超高效液相色谱-质谱技术 

分 类 号：S567.3[农业科学—中草药栽培]